Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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The Influence of Temperature Pretreatment on the Production of Microspore Embryos in Anther Culture of Capsicum annuum L.

고추 (Capsicum annuum L.)의 약배양 시 온도 전처리가 소포자배 발생에 미치는 영향

  • Published : 1999.03.01
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Abstract

Anthers of two hot pepper cultivars, Milyang-jare and Geryongsan-jare, were cultured on MS medium containing 0.1 mg/L 2,4-D and 0.1 mg/L kinetin. The influence of pretreatment at 4$^{\circ}C$ and 32$^{\circ}C$ on induction of microspore embryo was investigated. Milyang-jare was superior to the Geryongsan-jare in microspore embryo induction. The 32$^{\circ}C$ pretreatment increased embryo induction compared to the 4$^{\circ}C$ pretreatment while the 4$^{\circ}C$ pretreatment stimulated callus induction. Microspore embryos were regenerated to plantlets in the same medium or hormone free medium at 32$^{\circ}C$ treatment but most embryos failed to develop directly into plantlets at 4$^{\circ}C$ treatment. The optimal period of the 32$^{\circ}C$ pretreatment was 3 days in Milyang-jare and 6 days in Geryongsan-jare. The 32$^{\circ}C$ pretreatment was essential for induction and growth of microspore embryo in pepper.

약배양에 대한 반응이 비교적ㆍ높은 것으로 알려진 고추의 두 품종 계룡산재래와 밀양재래의 약을 2,4-D와 kinetin이 각각 0.1 mg/L씩 첨가된 배지에 치상한 후 4$^{\circ}C$와 32$^{\circ}C$의 온도 전처리가 소포자배 발생에 미치는 영향을 조사하였다. 소포자배의 발생은 사용한 두 품종 중 밀양재래에서 높았다. 고온처리는 저온처리에 비해 소포자 배의 발생이 많았고 캘러스의 발생은 거의 없었으며 발생한 배는 동일 배지에서 또는 호르몬이 없는 배지에 옮겼을 때 대부분이 정상적인 유식물로 발달하였다. 저온처리에서는 캘러스의 발생이 많았고 발생한 배는 대부분이 비정상적으로 발달하였다. 소포자배 발생에 효과적인 고온처리 기간은 밀양재래에서는 3일, 계룡산재래에서는 6일이었다. 고추의 약배양 시 고온처리는 소포자배의 유기뿐만 아니라 유기된 배의 생장에도 중요한 영향을 미치는 것으로 나타났다.

Keywords

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