Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지

Introduction of rolC gene into Petunia hybrida

Petunia hybrida 세포내로의 rolC 유전자의 도입

  • Published : 1999.01.01
Download PDF
⟨ Previous Next ⟩

Abstract

These experiments were attempted to introduce rolC gene in the Petunia hybrida cv. Titan white by Agrobacterium mediated. The maximum frequency of shoot regeneration was obtained by 60% on MS medium containing 1.0 mg/L BA, 0.1 mg/L NAA, 200 mg/L kanamycin, 500 mg/L carbenicillin, 30 g/L sucrose, and 8 g/L agar. Kanamycin-resistant calli were selected from petunia leaf discs by cocultivation with Agrobacterium suspension cultures on MS medium. The addition of AgNO$_3$ and KMnO$_4$ in the medium increased the shoot regeneration by 31.3% from leaf disc as compared with non-treated leaf disc. Among clones exhibiting kanamycin resistance, only 3 clones were confirmed by southern hybridization analysis.

쌍떡잎식물체 형질전환에 널리 쓰이는 Agrobacterium의 binary vector를 이용하여 왜화성을 나타내는 pGA643-rolC gene을 엽절편 transformation방법으로 petunia에 도입하였다. Petunia hybrida의 재분화에 있어 엽조직으로부터 식물체 재분화에 미치는 생장조절물질의 효과는 0.1mg/L NAA와 1.0mg/L BA의 조합에서 높았고 재분화된 식물체도 양호하였다. 식물체 형질전환 선발배지에 에틸렌 억제제인 AgNO$_3$와 KMnO$_4$의 첨가시 형질전환체 재분화율이 높게 나타났으며, AgNO$_3$에 비해 KMnO$_4$처리구에서 보다 많은 식물체 재분화율을 나타내었으나 AgNO$_3$와 KMnO$_4$의 고농도 첨가시 다소의 유리화 현상이 발생하였으므로 3mg/L KMnO$_4$ 첨가가 식물체 재분화에 적합한 것으로 생각된다. 항생제 200mg/L kanamycin, 500mg/L carbenicylin 과 1.0 mg/L BA, 0.1mg/L NAA가 첨가된 형질전환 선발배지에서 엽절편으로 부터 형질전환 된 것으로 추정되는 식물체들을 일차 선발하였고 기외로 이식한 후 genomic DNA를 분리하여 Southern blot analysis법으로 분석한 결과 외래 유전자가 식물체의 genomic DNA내로 삽입된 것을 확인할 수 있었다.

Keywords

References

  1. Kor J Plant Tiss Cult v.23 Development of bialaphos-resistant Petunia hybrida by introduction of the bar gene using Agrobacterium tumefaciens Aeom SI;Park SK;Lim YP;Lee CH;Kim HJ;Kim HR;Lee HY
  2. Plant Molecular Biology mannual v.ⅠA3 Binary vectors An G;Ebert PR;Mitra A;Ha SB;Gelvin SB(ed.)Schilperoort RA(ed.);Vema DS(ed.)
  3. Plant Cell Rep v.13 Agrobacterium-meediated transformatio of apple (Malusx domestica Borkh): An assessment of factors affecting gene transfer efficiency during early transformation steps Bondt AD;Eggmont K;Druart P;Vil MD;Goders I;Vandereyden J;Broekaert WF
  4. Plant Cell Rep v.9 Effect of AgNO₃and aminoethoxyvinylglycine on in vito shoot and root organo-genesis from seedling explants of recalcitrant Brassica genotypes Chi GL;Barfield DG;Sim GE;Pua EC
  5. Plant Cell Rep v.10 Stimulation of shoot regeneration from cotyledon of Helianthus annuus by the ethylene inhibitor, silver and cobalt Chraibi BK;Latch A;Roustan JP;Fallot J
  6. Kor J Plant Tiss Cult v.19 Putative GUS(β-glucuronidase) gene transfer into Petunia hybrida using Agrobacterium tumefaciens Chung JD;Kim CK;Kwon MY;Jee So
  7. Mol Gen Genet v.206 Genetic transfomation of oilseed rape (Brassica napus) by the Ri-T-DNA of Agrobacterium rhizogenes and analysis of inheritance of the transformed phenotype Guerche P;Jouanin L;Tepfer D;Pelletier G
  8. Sci. v.223 A simple and general method for tansferring genes into plants Horsch RB;Fry JE;Hoffmann NL;Eichholtz D;Rodgers SG;Fraley RT
  9. Plant Cell Tiss Org Cult v.32 Restoring adventitious shoot formation on chrysanthmum leaf explants following cocultivatio with Agrobacterium tumefaciens Jong JD;Rademaker W;Van Wordragen MF
  10. Graduate School of Kyungpook Nat;l Univ Drawf gene transfer into Petunia hybrida using vector system Kim HS
  11. Kor J Plant Tiss Cult v.24 Transformatio of Brassica napus via Agrobacterium vector:Plant regeneration and progeny analysis Kim KM;Sohn JK;Chung JD
  12. The Plant Cell v.2 Flavonoid genes in Petunia: Addition of a limited number of gene copies may lead to a suppression of gene expression Krol A;van Mur DLA;Beld M;Mol JNM;Stuitje AR
  13. Plant Cell Rep v.12 Promotion of flowering and morphological alterations in Atropa belladonna transformed with a CaMV 35S-rolC chimeric gene of the Riplasmid Kurioka Y;Suzuki Y;Kamada H;Harada H
  14. Theor Appl Genet v.88 Genetic transformation and regeneration of transgenic plants in grapevine(Vitis rupestris S.) Martinelli L;Mandolino G
  15. Plant Physiol v.15 A revised medium for rapid growth and bioassays with tobacco tissue culture Murashige T;Skoog F
  16. Nucleic Acids Res v.8 Rapid isolation of high molecular weight plant DNA murray MG;Thompson WF
  17. Jap J Genet v.65 Early flowering in transgenic tobacco plants possessing the rolC gene of Agrobacterlum rhizogenes Ri plasmid Oono Y;Kanaya K;Vchimiya H
  18. Mol Clen Genet v.199 Direct gene transfer to cells of a graminaceous monocot Potrykus I;Soul MW;Petruska J; Pazkowsk J;Shllllto RD
  19. Plant Cell Rep v.11 Genetic transofomation of sweet potato by particle bombardment Prakash CS;Varadarjan V
  20. Plant Cell Rep v.8 Stimulation of Daucus carota somatic embryogenesis by inhibitors of ethylene synthesis: cobalt and nickel Roustan JP;Latche A;Fallot J
  21. Molecular Cloning, A Laboratory Manual Sambrook JE;Fritsch EF;Maniatis T
  22. Mol Gen Genet v.152 Regeneration of plants from habituated and Agrobacterium transformed single-cell clones of tabacco Socristan MD;Melchers G
  23. J Mol Biol v.98 Detection of specific sequences among DNA fragments separated by gel electrophoresis Southem EM
  24. J Bateriol v.141 Hairy root:plasmid encodes virulence traits in Agrobacterium rhizogenes White FF;Nester EW
  25. Plant Cell Rep v.11 Expression of the GUS-gene in the monocot tulip after introduction by particle bombardment and Agrobacterium Wilmink A;Van De Ven BCE;Dons JJM
  26. Plant Cell Rep v.11 Agrobacterium tumefaciens-mediated transformation of safflower (Carthamus tinctorius L.) cv.'Centennial' Ying M;Dyer WE;Bergman JW