The Genetic Relationship of Zelkova Serrata Registered s the Natural Monument Using RAPD Markers

RAPD marker로 추적한 천연기념물로 지정된 느티나무의 유연관계

  • 강경홍 (전주대학교 생명과학부) ;
  • 정영재 (전주대학교 대학원 생물학과) ;
  • 김홍남 (전주대학교 자연과학연구소)
  • Published : 1999.03.01

Abstract

The genetic polymorphism and relationship among 14 Zelkova serrata registered as the natural monument in Korea were investigated using RAPD markers. N-J tree indicated that individuals in Kangwon-do were clustered and those in Yangam-gun and Damyang-gun of Chollanam-do were done closely. However, all the others were not agreed with the geographical distribution. Maybe this discordance was resulted from their movement by human being than any biological factors. The polymorphic percentages among individuals were from 77.8 to 100. From the high polymorphism, it was supposed that existing natural monuments were independently originated in the ancestors of long differentiated populations.

천연기념물로 지정된 14개체의 느티나무의 유연관계 및 개체간의 다양성을 RAPD 마커를 이용하여 조사하였다. 일반적으로 각 개체간의 유사성의 정도는 낮았고 강원도의 두 개체(KWH 와 KWS)간에서 78%로 가장 높은 유사성을 보였다. Neighbour-joining tree에서 보여진 유연관계는 강원도와 전남의 일부 개체를 제외하고는 지리적 분포와 일치하지 않았다. 이는 생물학적 요인이라기보다는 이 종의 인위적인 이동에 의한 결과로 사료된다. 또한 개체간의 유전적 polymorphism의 정도는 매우 높아 polymorphic band수의 퍼센트는 77.8%에서 100%였다. 이는 각 개체가 장기간 격리 분화된 조상형에서 유래되었던 결과로 추측되었다.

Keywords

References

  1. 한국기층문화의 탐구-6 마을숲-한국전통부락의 당숲과 수구막이 김학범;장동수
  2. 천연기념물 임경빈
  3. Heredity v.69 Detection of genetic variation between and with in populations of Gliricidia sepium and G. maculata using RAPD markers Chalmers KJ;R.Waugh;JI Sprent;AJ Simons;W Powell
  4. Theor. Appl. Genet. v.84 Potential taxonomic use of random amplified polymorphic DNA(RAPD): a case study in Brassica Demeke T;RP Adams;R Chibbar
  5. Focus v.12 Isolation of plant DNA from fresh tissue Doyle JJ;JL Doyle
  6. Theor. Appl. Genet. v.95 Pedigree assessment using RAPD-DGGE in cereal crop species Dweikat I;S Mackenzie;M Levy;H Ohm
  7. Jpn. J. Genet. v.67 Use of random amplified polymorphic DNA s(RAPDs)for identification of rice accessions Fukuoka S;K Hosaka;O Kamijima
  8. Theor. Appl. Genet. v.85 Inheritance of RAPDs in $F_1$hybrids of corn. Heun M;T Helentjaris
  9. Theor. Appl. Genet. v.85 Genetic variztion in agronomically important species of Stylosanthes determined using random amplified polymorphic DNA markers Kazan K;JM Mannera;DF Cameron
  10. J. Plant. Biol. v.40 Morphological and random amplified polymorphic DNA features in populations of Ceramiumkondoi(Rhodophyta) Kim Sun Ran;Su Yoon;Hwan;Min Boo Sung;Gi Paik Sang
  11. Mol. Gen. Genet. v.238 Construction of a basic genetic map for alfalfa using RFLP, RAPD, isozyme and morphological markers Kiss GB;G Casanadi;K Kalman;P Kalo;L Ikresz
  12. Theor. Appl. Genet. v.85 Identification of apple cultivars using RAPD markers Koller B;A Lehmann;JM McDermott;C Gessler
  13. Cultivated broad-leaved tree and shrubs Krussmann G
  14. Mol. Biol. Evol. v.7 no.5 The similarity index and DNA fingerprinting Lynch M
  15. Mol. Biol. Evol. v.4 The neighbor-joining method: a new method for reconstructing phylogenetic trees Saitou N& M Nei
  16. Theor. Appl. Genet. v.85 Random amplified polymorphic DNA and pedigreerelationships in spring barley Tinker NA;MG Gortin;DE Mather
  17. Flowering Plants, Dicotyledous Ulmaceae in The Families and Genera of Vascular Plant. Ⅱ. Todzia CA;K. Kubitzki(ed.)
  18. European Molecular Biology Laboratory Meyerhofstrasse 1 D 69117 Heidelberg Multiple alignment program Thompson JD;DG Higgins;TJ Gibson
  19. Theor. Appl. Genet. v.84 Use of RAPD markers to determine the genetic diversity of diploid, wheat genotypes Vierling RA;HT Nguyen
  20. Nucleic Acids Research v.18 no.2 DNA polymorphisms amplified by arbitrary primers are useful as genetic markers Williams GKJ;RA Kubelik;JK Livak;JA Rafalski;SV Tingey
  21. Theor. Appl. Genet. v.86 Identification and classification of celery cultivars with RAPD markers Yang X;C Quiros