Growth Characteristics of Tissue Cultured Plantlets by Lighting Direction and tight Intensity

광조사 방향 및 광도에 따른 배양식물체의 생장특성

  • 함인기 (충남농업기술원 부여토마토시험장) ;
  • 김현숙 (충남농업기술원 원예연구과) ;
  • 이미애 (충남농업기술원 원예연구과) ;
  • 조만현 (충남농업기술원 원예연구과) ;
  • 이은모 (충남농업기술원 원예연구과)
  • Published : 1999.06.01

Abstract

The present study was carried out to determine the effects of lighting directions and light intensities on the growth of in vitro plantlets of strawberry, potato and lily. The growth of plantlets was affected by two different lighting systems, downward and sideward lighting system. There were no difference for the plantlets of strawberry regardless of lighting directions. However, leaf area, fresh weight and dry weight have increased as the light intensity increased. Also, plant height and root length on node culture of potato decreased at high intensity of the sideward lighting, while thickness of stem and root was thicker, fresh weight and dry weight were heavier, and leaf area was increased. Also, bulblet formation on scale culture of lily has been abundant, and fresh weight and dry weight were increased. Thus, the sideward lighting system which loaded three stories for culture vessels with raising light intensity into culture vessels instead of conventional downward lighting system promoted growth and raised the efficiency of production of high quality microplant.

광조사 방향 및 광도에 따른 몇 가지 배양식물체의 생장반응을 조사하였다. 광조사 방향은 배양실내 조명장치를 상방향 또는 측방에 설치하여 조사하였다. 딸기 배양식물체의 초장과 근장은 광조사 방향에 관계없이 차이가 없었으나, 엽면적, 생체중 및 건물중은 광도가 높을 수록 증가하였다. 감자 절간 배양에서도 광도가 높은 측방광 조사구에서 초장이나 근장은 감소하였으나, 줄기 및 뿌리는 굵기가 굵고 생체중과 건물중 그리고 엽면적도 증가하였다. 백합인편배양 역시 측방광 고광도 조사구에서 자구 형성이 많았고 생체중과 건물중 모두 증가하였다. 따라서 관행 하방광 조사보다 배양용기의 측면에서 광을 조사하여 광도를 높이고, 배양용기를 3단으로 적재할 수 있는 측방광 조사방법이 배양식물체의 생장을 촉진시켜 양질 배양묘 생산효율을 증대시켰다.

Keywords

References

  1. Automation and environmental control in plant tissue culture Physical microenvironment and its effects Fujiwara, K.;Kozai, T.;J. Aitken-Christie(ed.);T. Kozai(ed.);ML Smith(ed.)
  2. Acta Hort v.319 Effect of sideward lighting on the growth of potato plantlets in vitro Hayashi, M.;Fujita, N.;Kitaya, Y.;Kozai, T.
  3. Horticultural Reviews v.17 Environmental control and photoautotrophic micropropagation Jeong, B. R.;Fujiwara, K.;Kozai, T.
  4. Scientia Horticulture v.62 Effects of light intensity and light direction on the photoautotrophic growth and morphology of potato plantlets in vitro Kitaya, Y.;Fukuda, O.;Kozai, T.
  5. Symposium of environmental effects and their control in plant tissue culture Environmental effects and their control in axillary bud multiplication Kitaya, Y.;Kozai, T.
  6. Automation and environmental control in plant tissue culture Environmental control in plant tissue culture -general introduction and overview Kozai, T.;Smith, M. L.;J Aitken-Christie(ed.);T. Kozai(ed.);ML Smith(ed.)
  7. Physiol Plant v.15 A revised medium for rapid growth and bioassays with tobacco tissue cultures Murashige, T.;Skoog, F.
  8. Kor J Hort Sci & Tech v.16 High quality microplant production and reduction of production cost through plant tissue culture Paek, K.Y.;Seon, J.H.;Son, S.H.;Han, B.H.;Yae, B.W.