Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Molecular Cloning and Characterization of a Heat Shock Protein 70-related cDNA from Olive flounder (Paralichthys olivaceus)

  • Kim, Woo-Jin (Biotechnology Division, National Fisheries Research and Development Institute) ;
  • Lee, Jeong-Ho (Biotechnology Division, National Fisheries Research and Development Institute) ;
  • Kim, Kyung-Kil (Biotechnology Division, National Fisheries Research and Development Institute) ;
  • Lee, Sang-Jun (Biotechnology Division, National Fisheries Research and Development Institute) ;
  • Kang, Ho-Sung (Department of Molecular Biology, Pusan National University) ;
  • Kim, Han-Do (Department of Molecular Biology, Pusan National University)
  • Published : 1999.06.01
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Abstract

The complete nucleotide sequence of olive flounder (Paralichthys olivaceus) hsp70-related rDNA was determined by RT- and RACE-PCR methods. A full-length of hsp70-related cDNA has an open reading frame of 1.95 kb encoding 650 amino acids with a calculated molecular weight of 71.1 kD. A corresponding hsp70-related protein contains a number of conserved elements including an ATP-binding domain, a nuclear localization signal and the carboxyl terminal motif, EEVD, which may have a role in chaperone function. Comparison of nucleotide and predicted amino acid sequence between olive flounder hsp70-related gene and hsp/hsc70 genes of other species revealed a high similarity with the cognate form of these genes. These results indicated that we recovered likely to be a olive flounder cognate hsc70 gene.

Keywords

References

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