Journal of Photoscience

Korean Society of Photoscience (한국광과학회)
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EFFECTS OF ETHANOLON NMDA-MEDIATED INTRACELLULAR FREE $Ca^{2+}$ CONCENTRATION IN DISSOCIATED BRAIN CELLS

  • Chung, In-Kyo (Departments of Clinical Pharmacology and Maxillofacial Surgery College of Dentistry and Research Institute for Oral Biotechnology Pusan National University) ;
  • Kim, Dong-Soo (Departments of Dental Pharmacology and Biophysics, College of Dentistry and Research Institute for Oral Biotechnology Pusan National University) ;
  • Chung, Yong-Za (Department of Biochemistry, College of Pharmacy, Kyungsung University) ;
  • Kim, Inn-Se (Departments of Anesthesiology, College of Medicine and Research Institute for Oral Biotechnology, Pusan National University) ;
  • Cho, Goon-Jae (Departments of Internal Medicine, College of Medicine and Research Institute for Oral Biotechnology, Pusan National University) ;
  • Park, Chang-Hwa (Departments of Neurosurgery, College of Medicine and Research Institute for Oral Biotechnology, Pusan National University) ;
  • Kim, Bong-Sun (Departments of Anatomy, College of Medicine and Research Institute for Oral Biotechnology, Pusan National Unversity) ;
  • Jang, Hye-Ock (Department of Oriental Pathology and Prescription, College of Oriental Medicine, Dong-Eui University) ;
  • Il Yun (Departments of Dental Pharmacology and Biophysics, College of Dentistry and Research Institute for Oral Biotechnology, Pusan National University)
  • Published : 1999.12.01
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Abstract

Using fluorescent probe fura-2 acetoxymethyl ester, we studied effects of N-Methyl-D-aspartate (NMDA) on free intracellular $Ca^{2+}$ concentration ([$Ca^{2+}$]$_{i}$) and interaction of ethanol with NMDA-mediated response in freshly dissociated brain cells from newborn rats. Twenty five micromolar NMDA significantly increased ($Ca^{2+}$), and this increasing effect could be prevented or reversed by the NMDA antagonists $Mg^{2}$(1.0 mM) and 2-amino-5-phosphonovalerate (AP5, 100 ${\mu}$M). Ethanol at concentrations from 2.5 to 100 mM inhibited NMDA-mediated calcium current in a concentration-dependent manner. Maximal inhibition of NMDA-mediated calcium current by ethanol was 82% at 50 mM. The ethanol inhibition at 100 mM was not significantly different from the inhibition at 50 mM.

Keywords

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