Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
권호 표지

Genetic Differentiation in the Mitochondrial Cytochrome b Gene of Korean Brown Frog, Rana dybowskii (Amphibia: Ranidae)

  • Kim, Yu-Ri (Department of Biology, College of Sciences, Inha University) ;
  • Yang, Dong-Eun (Department of Biology, College of Sciences, Inha University) ;
  • Lee, Hyuk (Department of Biology, College of Sciences, Inha University) ;
  • Lee, Jung-Eun (Department of Biology, College of Sciences, Inha University) ;
  • Lee, Hyun-Ick (Department of Biology, College of Sciences, Inha University) ;
  • Yang, Suh-Yung (Department of Biology, College of Sciences, Inha University) ;
  • Lee, Hei-Yung (Department of Biology, College of Sciences, Inha University)
  • Published : 1999.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

The nucleotide sequences of a 504 bp segment of the mitochondrial cytochrome b gene were analyzed to survey the intraspecific variation of the brown frog, Rana dybowskii, collected from nine populations in South Korea. Comparisons of sequence divergence of the cytochrome b gene suggest that the populations examined are clearly classified into two types (type 1 and type 2), diverged from each other by a high value of 14.3-15.9% sequence divergence. The two types are distributed allopatrically in most populations, but only one population occurs sympatrically. In the Tonghae population, their spawning grounds differ in that type 1 spawns in the puddle and type 2 spawns in the mountain creek. Based on the genetic divergences of the cytochrome b gene sequences, the phylogenetic status of Korean R. dybowskii is elucidated by comparing it with related brown frogs distributed in an area adjacent to the Korean Peninsula. Interspecific sequence divergences among type 1, type 2 and other related brown frog species (Russian R. dybowskii, R. pirica, R. ornativentris, R. chensinensis: 2n=24 chromosomes) used in this study ranged from 11.7 to 16.3%. R. dybowskii in Tsushima is very similar to our type 1 (sequence divergence＝0-1.6%) and R. chensinensis in western China is closest to our type 2 (sequence divergence＝6.8-7.5%).

Keywords

References

  1. Mol Phylogenet Evol v.9 Francolin phylogenetics: molecular, morphobehavioral, and combined evidence Bloomer P;Crowe TM
  2. Gene v.36 nucleotide sequence of a Xenopus laevis mitochondrial DNA fragment containing the D-loop, flanking tRNA genes and apocytochrome b gene Dunon-Bluteau D;Volovitch M;Brun G
  3. Copeia v.1998 Influence of the Guadalquivir river basin on mitochondrial DNA evolution of Salamandra salamandra (Caudata: Salamandridae) from southern Sapin Garcia-Paris M;Alcobendas M;Alberch P
  4. Zool J Linn Soc v.109 Evolutionary relationships of eastern Palearctic brown frogs, genus Rana: paraphyly of the 24-chromosome species group and the significance of chromosome number change Green DM;Borkin LJ
  5. Mol Biol Evol v.15 A comparative summary of genetic distances in the vertebrates from the mitochondrial cytochrome b gene Johns GC;Avise JC
  6. Illustrated Encyclopedia of Fauna and Flora of Korea. Vol 17. Amphibia and Reptilia Kang YS;Yoon IB
  7. J Mol Evol v.16 A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences Kimura M
  8. Proc Natl Acad Sci USA v.86 Dynamics of mitochondrial DNA evolution in animals: amplication and sequencing with conserved primers Kocher TD;Thomas WK;Meyer A;Edwards SV;Paabo S;Villablanca FX;Wilson AC
  9. MEGA: Molecular Evolutionary Genetics Analysis(Version 1.01) Kumar S;Tamura K;Nei M
  10. Korean J Genet v.19 Genetic differentiation between two species in the genus Rana of Korea Lee HY;Oh SJ;Yang DE;Kim YR;Yang SY
  11. Frogs and Toads of Japan Maeda N;Matsui M
  12. Jpn J Herpetol v.17 Phylogenetic relationships among local populations of Rana dybowskii assessed by mitochondrial cytochrome b gene sequences Matsui M;tanaka-Ueno T;Paik NK;Yang SY;Takenaka O
  13. Mol Biol Evol v.14 Phylogenetic relationships of artiodactyls and cetaceans as deduced from the comparison of cytochrome b and 12S rRNA mitochondrial sequences Montgelard C;Catzeflis FM;Douzery E
  14. MS Thesis, Inha University The analysis of partial sequences of cytochrome b gene in four species of Korean Rana Oh SJ
  15. J Chosen Nat Hist Soc v.6 Frogs in Korea Okada Y
  16. Molecular Cloning: a Laboratory Manual(2nd Ed.) Sambrook J;Fritsch EF;Maniatis T
  17. Herpetologica v.12 The reptiles and amphibians of Korea Shannon FA
  18. Zool Sci v.13 Genetic variation and population divergence in the mountain brown frog Rana ornativentris Sumida M;Nishioka M
  19. Mol Biol Evol v.10 Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in human and chimpanzees Tamura K;Nei M
  20. Mol Phylogent Evol v.4 Mt DNA phylogeography of the Califjornia Newt, Taricha torosa (Caudata, Salamandridae) Tan AM;Wake DB
  21. Zool Sci v.11 Estimation of phylogenetic relationships among japanese brown frogs (Amphibia: Anura) from mitochondrial cytochrome b gene Tanaka T;Matsui M;Takenaka O
  22. Biochem Syst Ecol v.24 Phylogenetic relationships of Japanese brown frogs (Rana: Ranidae) assessed by mitochondrial cytochrome b gene sequences Tanaka T;Matsui M;Takenaka O
  23. Zool Sci v.15 Phylogenetic relationships of brown frogs (Rana: Ranidae) with 24 chromosomes from far east Russia and Hokkaido assessed by mitochondrial cytochrome b gene sequences Tanaka-Ueno T;Matsui M;Sato T;Takenaka S;Takenaka O
  24. Mol Biol Evol v.14 Phylogenetic relationships of bolitoglossine salamanders: a demonstration of the effects of combining morphological and molecular data sets Todd RJ;Applebaum G;Wake DB
  25. Check list of Korean amphibians v.5 Yang SY;Yu CH
  26. Basic Sci Res Inst Inha Univ, Inchon v.1 Osteology of Korean anurans Yu CH;Yang SY
  27. Herpetology of China Zhao EM;Adler K
  28. Auk v.113 Patterns of allozyme, mitochondrial DNA, and morphometric variation in four sparow genera Zink RM;Blackwell RC