Animal cells and systems

  • 제3권1호
  • /
  • Pages.69-72
  • /
  • 1999
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  • 1976-8354(pISSN)
  • /
  • 2151-2485(eISSN)
한국통합생물학회 (The Korean Society for Integrative Biology)
권호 표지

Identification of a Tandemly Repented DNA Sequence Using Combined RAPD and FISH in Welsh Onion (Allium fistulosum)

  • Bong Bo Seo (Department of Biology, College of Natural Sciences, Kyungpook National University) ;
  • Geum Sook Do (Department of Biology, College of Natural Sciences, Kyungpook National University) ;
  • Seon Hee Lee (Department of Biology, College of Natural Sciences, Kyungpook National University)
  • 발행 : 1999.03.01
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초록

A tandemly repeated DNA sequence was identified and characterized y the combined RAPD and FISH data from a total genomic DNA of Welsh onion (Allium fistulosum). A clone containing this repeating sequence was selected and sequenced. This repeating unit of 314 bp inserted into pAf 072 contained 54.1% adenine and thymine residues, and showed the primer sequence used, 5'-GAAACGGGTG-3', in both terminals of the sequence. Fluorescence in situ hybridization using this tandemly repeated sequence as a probe indicated that the detected sites were coincident with the major C-banded constitutive heterochromatin in the terminal regions of both arms of all 6 chromosomes.

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