Animal Systematics, Evolution and Diversity

The Korean Society of Systematic Zoology (한국동물분류학회)
권호 표지

Variation Pattern of mtDNA among Six Subspecies of Apodemus agrarius(Mammalia, Rodentia) in Korea, China, and Russia

한국, 중국, 러시아에 서식하는 등줄쥐, Apodemus agrarius(포유강, 설치목), 6아종의 미토콘도리아 DNA 절단단편의 변이

  • Published : 1999.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

One hundred and eleven samples of six subspecies of striped field mouse, Apodemus agrarius Pallas from Korea, China and Russia, were used for the analysis of mitochondrial DNA(mtDNA) fragment patterns resulted from the digestion with eight restriction enzymes by blot hybridization technique. All 32 fragments, nine mtDNA haplotypes, and four major subgroups with the mean divergence value of 0.896 to 1.150% were revealed. In summary, three forms are recognized: [I, subspecies chejuensis (Chejudo island, Korea)], [II, subspecies pallescens (southwestern Korea), coreae (central Korea), and septentrionalis (Russia)], and [III, subspecies manchuricus (northeastern China) and pallidior (northern China)], although some samples of subspecies coreae are somewhat different from almost all samples of six subspecies, and some samples of subspecies pallidior are similar with all samples of subspecies septentrionalis to form same haplotype. It is confirmed that A. agrarius chejuensis is a distinct subspecies, that subspecies coreae (including pallescens) is also a distinct subspecies, that subspecies manchuricus and pallidior are synonyms of subspecies ningpoensis, and that subspecies septentrionalis is a synonym of subspecies ningpoensis, and that subspecies septentrionalis is a synonym of subspecies agrarius. Moreover, it seems that A. agrarius shows constant karyotype, minimal variation in mtDNA genotype, and considerable divergence in morphometric characters, although further analyses with additional samples of A. agrarius in Eurasia will be necessary to determine the degree of variation of these taxonomic characters and to clarify subspecies classification as well.

한국, 중국, 러시아에 서식하는 등줄쥐 Apodemus agrarius 6아종 111마리를 이용하여, 미토콘드리아 DNA를 8개의 제한효소로 절단한 단편들을 blot hybridization법으로 분석하였다. 모두 32개의 단편들이 보여졌고, 9개의 haplotype이 밝혀졌으며, 평균 발산값이 0.896에서 1.150%인 4아군이 나타났다. 결론적으로 세 형을 인정할 수가 있었다: [I. 아종 chejuensis (제주도, 한국)], [ll, 아종 pallescens(남서 한국), coreae(중앙 한국), 및 septentrionalis(러시아)], and [lll, 아종 manchuricus(북동 중국)와 pallidior(북부 중국)]. 그러나 아종 coreae의 일부 표본들은 다른 6아종의 표본들과 다소 차이를 보였고, 아종 pallidior의 일부 표본들은 아종 septentrionalis의 모든 표본들과 같아서 동일한 haplotype을 형성하였다. 아종 a. agrarius chejuensis는 독특한 아종중의 하나이고, 아종 corea(pallescens포함)도 독특한 아종중의 하나이며, 아종 manch-uricus와 pallidior는 아종 ningpoensis의 동아종이명이고, 아종 septentrionalis도 아종 agrarius의 동아종이명임이 재확인되었다. 뿐만 아니라, 등줄쥐는 염색체 핵형이 일정하며, 미토콘드리아 DNA 유전형에 약간의 변이를 보이고, 형태형질의 상당한 발산을 보이고 있지만, 이들 분류형질의 변이 정도의 파악과 아종분류의 재검토를 위하여 유라시아산 등줄주의 보다 많은 표본을 이용한 분석이 앞으로 필요하다.

Keywords

References

  1. Reithrodontomys megalotis. J. Mamm. v.70 Absence of differentiation in mitochondrial DNA of island and mainland harvest mice Ashley, M. V.
  2. Phil. Trans. R. Soc. Lond. v.312 Mitochondrial DNA and the evolutionary genetics of higher animals Avise, J. C.
  3. Genetics v.97 Intra- and interspecific variation of the mitochondrial genome in Rattus norvegicus and Rattus rattus: restriction enzyme analysis of variant mitochondrial DNA molecules and their evolutionary relationships Brown, G. G.;M. V. Simpson
  4. The mammals of the Palaearctic region: a taxonomic review. British Museum(Nat. Hist.) Corbet, G. B.
  5. Ph.D. Dissert. Washington Univ. Evolutionary relationships in Geomys: an analysis using mitochondrial DNA Davis, S. K.
  6. TREE v.13 Molecules remodel the mammalian tree De Jong, W. W.
  7. Molecular systematics Nucleic acids Ⅱ: restriction site analysis Dowling, T. E.;C. Moritz;J. D. Palmer;Hills, D. M.(eds.);C. Moritz(eds.)
  8. Molecular systematics Hills, D. M.;C. Moritz;B. K. Mable
  9. TREE v.11 Combining data in phylogenetic analysis Huelsenbeck, J. P.;J. J. Bull;C. W. Cunningham
  10. Proc. Biol. Soc. Washington v.68 Three new rodents of the genera Micromys and Apodemus from Korea. Johnson, D.H.;J.K. Jones
  11. Synopsis of the lagomorphs and rodents of Korea v.16 Jones, J. K.;D. H. Johnson
  12. Contemporary mammalogy in China and Japan Taxonomic problems in the genus Apodemus and its allies Kobayashi, T.;Kawamichi, T.(ed.)
  13. Korean J. Zool. v.25 G- and C-banding pattern analyses of Korean rodents: I. Chromosome banding patterns of striped field mice, Apodemus agrarius coreae, and black rats, Rattus rattus rufescens Koh, H.S.
  14. Korean J. Zool. v.29 Geographic variation of morphometric characters among three subspecies of striped field mice, Apodemus agrarius Pallas from Korea Koh, H. S.
  15. Cheju-Do. Korean J. Syst. Zool. v.3 Morphometric and chromosomal analyses of striped field mice, Apodemus agrarius chejuensis Jones and Johnson Koh, H. S.
  16. Korea. Korean J. Syst. Zool. v.7 Multivariate analysis with morphometric characters of samples representing eight subspecies of striped field mice, Apodemus agrarius Pallas, in Asia: taxonomic status of subspecies chejuensis at Cheju island Koh, H. S.
  17. Korea. Korean J. Zool. v.35 Variation of mitochondrial DNA in two subspecies of striped field mice, Apodemus agrarius coreae and A. agrarius chejuensis Koh, H. S.;B. S. Yoo
  18. Korean J. Syst. Zool. v.14 Morphometric analyses with 15 subspecies of striped field mouse, Apodemus agrarius Pallas (Mammalia, Rodentia) from Eurasia Koh, H. S.;G. Tikhonova
  19. Zool. Listy. v.19 Chromosome strudies in two subgenus of the genus Apodemus. Kral, B.
  20. Peromyscus maniculatus. Evolution. v.37 Extensive genetic variation in mitochondrial DNA's among geographic population of deer mice Lansmann, R. A.;J. C. Avise;C. F. Aquadro;J. F. Shapira;S. W. Daniel
  21. Acta Theriol. Sinica v.11 A study of the subspecies classification of Apodemus agrarius in eastern continental China. Liu, C.;W. Wu;S. Guo;J. Meng
  22. Principles of systematic zoology Mayr, E.
  23. Principles of systematic zoology Mayr, E.;P. D. Ashlock
  24. Rodentia of the north Caucasus Ognev, S. I.
  25. Evolution v.51 Correlated rates of molecular and morphological evolution Omland, K. E.
  26. Molecules and morphology in evolution Introduction Patterson, C.;Patterson, C.(ed.)
  27. Principles and techniques of contemporary taxonomy Quicke, D. L. J.
  28. Numerical taxonomy Sneath, P. H. A.;R. R. Sokal
  29. J. Mol. Evolution v.98 detection of specific sequences among DNA fragments separated by gel electrophoresis Southern, E. M.
  30. Hereditas v.111 Genetic divergence in mitochondrial DNA between wood mice(Apodemus sylvaticus) and the yellow necked mice(A. flavicolis) Tegelstrom, H.;M. Jaarola
  31. Biochem. Genetics v.26 Concordant divergence in proteins and mitochondrial DNA between two vole species in the genus Clethrionomys Tegelstrom, H. P.;Wyoni;H. Gelter;M. Jaarola
  32. N. China. Proc. Zool. Soc. London The Duke of Bedford's zoological exploration in eastern Asia: Ⅵ. List of mammals from the Shantung peninsula Thomas, O.
  33. Nucleic Acids Res. v.4 Estimation of DNA sequence divergence from comparison of restriction endonuclease digests Upholt, W. P.
  34. Mamm. Soc. Japan Subspecific classification and distrubution of Apodemus agrarius in Sichuan, China, In: Contempory mammalogy in China and Japan Wang, Y.;Kawamichi, T.(ed.)
  35. Acta Theriol. Sinica v.17 Mitochondrial DNA restriction fragment patterns by blot hybridization technique in two subspecies of striped field mice, Apodemus agrarius Pallas(Mammalia, Rodentia) Wang, J.;H. S. Koh
  36. Acta Theriol. Sinica v.13 Studies of chromosome of striped field mouse, Apodemus agrarius pallidior(Rodentia) Wang, J.;X. Zhao;X. Wang;J. Tian
  37. Syst. Zool. v.2 The subspecific concept and its taxonomic application. Wilson, E. O.;W. L. Brown
  38. Acta Theriol . Sinica v.4 A study on Chinese Apodemus with a discussion of its relations to Japanese species Xia, W.
  39. Genetics v.98 Evolutionary relationships among five subspecies of Mus musculus based on restriction enzyme cleavage patterns of mtDNA. Yonekawa, H.;K. Morowaki;O. Gotoh;J. Hayashi;J. Watanabe;N. Miyashita;M.L. Petras;Y. Tagashira
  40. Acta Theriol. Sinica v.6 Comparative observations of several biochemical indexes of Apodemus agrarius pallidior and Apodemus agrarius ningpoensis of the striped backed field mice Zhao, X.;H. Lu