파밤나방(Spodoptera exigua (Hubner))과 담배거세미나방(Spodoptera litura(Fabricius))에 대한 곤충병원선충(Steinernema carpocapsae Weiser)의 감염력 및 증식력

Pathogenicity and Multiplication of Entomopathogenic Nematode, Steinernema carpocapsae Weiser, on Been Armyworm, Spodoptera exigua (Hubner) and Tobacco Cutworm, Spodoptera litura(Fabricius)

곤충병원선충(Steinernema carpocapsae Weiser) 기주 증식을 위해 실내 대량사육이 용이한 파밤나방(spodoptera exigua (Hubner))과 담배거세미나방(Sp. litura (Fabricius))유충에서 병원성과 증식선충수를 조사하였다. 반수치사 병원선충수는 두 기주간 또는 발육시기에 따라 차이가 없었으나 기주내에 증식된 선충수는 기주의 크기에 비례하였다. 온도에 따른 선충의 증식은 온도가 증가함에 따라 기주내 최대 감염태 선충수(담배거세미나방 5령충에서 약 500,000마리, 파밤나방 5령충에서 약 100,000 마리)를 보이는 소요기간이 $25^{\circ}C$에는 접종 후 6일이나 $20^{\circ}C$에서는 12일로 길어졌다.

Pathogenicity and multiplication of entomopathogenic nematode, Steinernema carpocapsae Weiser, ere analyzed in two insect hosts, Spodoptera exiga (Hubner) and Sp. litura(Fabricius). The estimated ${LC}^{50}s$(lethal concentration of the infective juveniles to kill 50% of the host insect population) were not different between tow insect species or among their developmental stages on the filter paper assay, thought the actual numbers of the infected nematodes were varied among them. The significant variation, however occurred in nematode multiplication between tow inset hosts. Temperature also gave sosignificatn effect on nematode multiplication rate that it took 6 days after infection at $25^{\circ}C$, but did 12 days at $20^{\circ}C$ to show the maximal nematode population peak($\approx$500,000 infective juveniles(IJ) in a 5th instar larva of Sp. litura and $\approx$-100,000 IJ in a 5th instar larva of Sp. exigua).