Antimutagenic Effects of Water Extracts of Persimmon Leaf Tea, Green Tea and Oolong Tea on Reversion and Survival of Selected Salmonella Tester Strains

Salmonella typhimurium Strain TA98, 100에서 감잎차, 녹차, 우롱차 추출물의 돌연변이 억제 효과

  • 강명희 (한남대학교 식품영양학과) ;
  • 송현순 (한국화학연구소 안정성 연구센터) ;
  • 이현걸 (한국화학연구소 안정성 연구센터)
  • Published : 1999.06.01


Water extracts of persimmon leaf tea(PLTE), green tea(GTE) and oolong tea(OTE), at the con centration used for human consumption, were examined for inhibitory effects on the mutagenicity of major classes of dietary and environmental mutagens including indirect acting mutagens, B[ ]P (benzo[ ]pyrene), IQ(2 amino 3 methylimidazo[4,5 f]quinoline), 2 AA(2 aminoanthracene) in the presence of S9 mix and direct acting mutagen, 4 NQO(4 nitroquinoline 1 oxide) without S9 mix, using the modified Ames Salmonella/microsome assay. PLTE, GTE and OTE showed very potent and concentration dependent antimutagenic effects against indirect acting mutagens B[ ]P and IQ. At the maximum concentration(16,200 g/plate) of each tea extract, number of colonies decreased in a dose dependent manner up to 82~100%. Similar inhibition of PLTE, GTE and OTE were seen at higher concentration in the mutagenicity of the 2 AA following an initial increase in the activity at lower concentration. However, the mutagenicity of the direct acting mutagen 4 NQO were not suppressed at lower concentration of the three tea extracts, and higher concentration of the tea extracts enhanced mutagenic activity of the mutagen. There were no differences in the mode of antimutagenesis between PLTE, GTE, and OTE, in both Salmonella typhimurium TA98 and TA100 strains against the same mutagen. In conclusion, the water extracts of persimmon leaf tea, green tea and oolong tea possess marked antimutagenic potential against a variety of important dietary and environmental indirect acting mutagens, but the activity was not observed against the direct acting mutagens. These results suggest that the mode of inhibitory action may not have resulted from direct interaction between tea extracts and the mutagens, but rather from indirect metabolic inactivation of mutagens by tea extracts.


  1. Cancer Res. v.48 The origins of human cancer: Molecular mechanisms of carcinogenesis and their implications for cancer prevention and treatment. Weinstein, I. B.
  2. Carcinogenesis v.9 Isolation and identification of mutagens from a fried norwegian meat product Becher, G.;Knize, M. G.;Nes, I. F.;Felton, J. S.
  3. Proceedings of the 23rd International symposium Geterocyclic amines in cooked foods Adamson, R. H.;Gustafsson, J. A.;Ito, N.;Nagao, M.;Sugimura, J.;Wakabayashi, K.;Yamazoe, Y.
  4. Mutation Res. v.53 Antimutagenic action of vegetable factors on the mutagenicity of the mutagenic principle of tryptophan pyrolysate Kada, T.;Morita, K.;Inoue, T.
  5. J. Agric. Food Chem. v.46 Hypolipidemic effect of green tea liaves through induction of antioxidant and phaseⅡ enzymes including superoxide dismutase, catalase, and glutathione s-transferase in rats Lin, Y. L.;Cheng, C. Y.;Lin, Y. P.;Lau, Y. W.;Juan, I. M.;Lin, J, K.
  6. CRC Crit. Rev. Food Sci. Nutr. v.12 the biochemistry and technology of tea manufacture Bokuchava, M. A.;Skobeleva, N. I.
  7. J. Cell Biochem. v.27 Cnemopreventive effect of green tea(Camellia sinensis) against cigarette smoke-induced mutations(SCE) in humans Lee, I. P.;Kim, Y. H.;Kang, M. H.;Roberts, C.;Shim, J. S.;Roh, J. K.
  8. Mutation Res. v.223 Antimutagenic activity of green tea polyphenols Wang, Z. Y.;Cheng, S. J.;Zhou, Z. C.;Athur, M.;Khan, W. A.;Bickers, D. R.;Mukhtar, H.
  9. J. Food Pro. v.57 Comparison of antimutagenic effect of various tea extracts(green, oolong, pouchung and black tea) Yen, G. C.;Chen, H. Y.
  10. Carcinogenesis v.14 Pretection against N-nitrosodiethlamine and benzo[α]pyrene-induced forestomach and lung tumorigenesis in A/J mice by green tea Katiyar, S. K.;Agarwal, R.;Zaim, M. T.;Mukhtar, H.
  11. Mutation Res. v.326 Screening of tea clones for inhibition of PhIP mutagenicity Apostolides, Z.;Weisburger, J. H.
  12. Biosci. Biotech. Beochem. v.58 Antimutagenic activity of water extracts of black tea and oolong tea Yamada, J.;Tomita, Y.
  13. J. Korean Soc. Food Nutr. v.25 Antimutagenic effects of persimmon leaf tea extracts in sister chromated exchange(SCE) assay system Song, H. S.;Lee, H. K.;Jang, H. D.;Kim, J. I.;park, O. J.;Lee, M. S.;Kang, M. H.
  14. Mutation Res. v.393 Possible mechanisms of antimutagens by various teas as jugsed by their effects on mutagenesis by 2-amino-3-methylimidazo [4,5-f]quinoline and benzo[α]pyrene Chen, H. Y.;Yen, G. C.
  15. Mutagenesis v.11 A comparison of the antimutagenic potential of green, black and decaffeinated teas: contribution of flavanols to the antimutagenic effect Bu-Abbas, A.;Nunez, X.;Clifford, M. N.;Walker, R.;Ioannides, C.
  16. Food Chem. Toxicol. v.34 The effect of teas on the in vitro mutagenic potential of heterocyclic aromatic amines Stavric, B.;Matula, T. I.;Klassen, R.;Downie, R. H.
  17. J. Agr. Food Chem. v.43 Antioxidant activity of various tea extracts in relations to their antimutagenicity Yen, G. C.;Chem, H. Y.
  18. Mutation Res. v.150 Detection and chemical identification of matural bio-antimutagens: A case of green tea factor Kada, T. K.;Kaneko, S.;Matsuzaki, T. M.;Hara, Y.
  19. Mutagenesis v.11 Relationship between antimutagenic activity and major components of marious teas Yen, G. C.;Chem, H. Y.
  20. J. Korean Soc. Food Nutr. v.24 Desmutagenicity of tea extracts from green tea, oolong tea and black tea Yeo, S. G.;Kim, I. S.;Ahn, C. W.;Kim, S. B.;Park, Y. H.
  21. J. Korean Soc. Food Nutr. v.24 Antimutagenic effects of boiled water extract and tannin from persimmon leaves Moon, S. H.;Park, K. Y.
  22. Mutation Res. v.113 Revised methods for the Salmonella mutagenicity test Maron, D. M.;Ames, B. N.
  23. Mutation Res. v.361 Bio-antimutagenic activity of green tea catechins in cultured Chinese hamster V79 cells Kuroda, Y.
  24. Environmental mutagens and carconogens Mechanisms and genetic implications of environmental antimutegens Kada, T.;Sugimura, T.;Kondo, S.;Takebe, H.(eds.)
  25. Mutation Res. v.286 The clastogen-suppressing effects of green tea, Po-lei tea and Rooibos tea in CHO cells and mice Sasaki, Y. F.;Yamada, H.;Shimoi, K.;Kator, K.;Kinae, N.