KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Fed-Batch Simultaneous Saccharification and Fermentation of Waste Paper to Ethanol

폐지의 유가식 동시당화발효에 의한 에탄올 생산

  • 권정기 (고려대학교 공과대학 화학공학과) ;
  • 문현수 (고려대학교 생명공학원) ;
  • 김준석 (고려대학교 공과대학 화학공학과) ;
  • 김승욱 (고려대학교 공과대학 화학공학과) ;
  • 홍석인 (고려대학교 공과대학 화학공학과)
  • Published : 1999.02.01
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Abstract

The fed-bach simultaneous saccharification and fermentation (SSF) of newspaper to ethanol with Brettanomyces custersii was studied. The initial substrate concentration for the effective fed-batch SSF was 8% (w/v). The initial optimum enzyme concentration was 30 FPU/g cellulose for cellulase and the optimum volumetric ratio of $\beta$-glucosidase to cellulase was 0.1. When 4% (w/v) of ball-milled newspaper was supplemented intermittently at time intervals, considering the mixing of newspaper slurry, the fed-batch SSF showed higher ethanol concentration (26.80 g/L) and two times higher ethanol production yield based on enzyme than the batch SSF.

Brettanomyces custersli를 이용하여 신문지의 유가식 동시 당화발효에 의한 에탄올 생산에 대한 연구를 수행하였다. 유가식 동시당화발효에 의한 에탄올 생산을 효과적으로 수행하기 위한 초기 기질 투입 농도는 8%(w/v)이었고 효소의 비용을 감안한 최적 효소 농도는 cellulase의 경우 30EPU/g cellulase, $\beta$-glucosidase와 cellulase의 부피가 0.1(활성비 1.05)이었다. 앞에서 결정된 초기 조건들을 가지고 교반식 반응기에서 수행된 유가식 동시당화발효는 26.8g/L의 에탄올을 생산성에서 회분식 동시당화발효보다 약 2배의 값을 나타내었다.

Keywords

References

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