Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Production of Lyopilized Culture of Lactobacillus acidophilus with Preserving Cell Viability

  • Kang, Moo-Heon (Bioprocess Eng. Lab., Hanhyo Institute of Technology, Taejon) ;
  • Vibhor Saraswat (Biochemical Process Engineering R. U., Korea Research Institute of Bioscience and Biotechnology, (KRIBB), Taejon) ;
  • Lee, Jeewon (Biochemical Process Engineering R. U., Korea Research Institute of Bioscience and Biotechnology, (KRIBB), Taejon) ;
  • Park, Young-Hoon (Biochemical Process Engineering R. U., Korea Research Institute of Bioscience and Biotechnology, (KRIBB), Taejon)
  • Published : 1999.03.01
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Abstract

Optimal lyophilization process was developed for manufacturing the dried product of Lactobacillus acidophilus with high cell viability. Three major factors, freezing rate, specific surface area of samples, and stabilizer type and their synergy were shown to play a crucial role in the development of an effective lyophilization process. Finally we found an optimal combination among three process parameters mentioned above; an exceptionally high cell survival percentage of 90% was achieved using the 8.28 cm-1 specific surface area of samples, slow freezing rate, and a stabilizer composition of 4% skim milk +1% glycerol +0.1% calcium chloride.

Keywords

References

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