한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제27권6호
  • /
  • Pages.466-470
  • /
  • 1999
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

재래식 메주로부터 분리한 Mucor racemosus f. racemosus PDA 103이 생산하는 Fungal Protease 특성

Characteristics of Fungal Protease Produced by Mucor racemosus f. racemosus from Korean Traditional Meju

  • 임성일 (한국식품개발연구원 생물공학연구부) ;
  • 유진영 (한국식품개발연구원 생물공학연구부)
  • 발행 : 1999.12.01
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초록

Protease production and its characteristics were investigated with Mucor racemosus f. racemosus PDA 103 which was isolated from Korean traditional meju. Optimum culture conditions of the strain for the production of the protease in basic medium[bean(Baektae):H2O=1:1(w/v)] were as follows: pH 6, 3$0^{\circ}C$ and 72hrs. Optimum pH and temperature for the enzyme activity of the protease produced by Mucor racemosus f. racemosus were pH 5 and 5$0^{\circ}C$, respectively. The enzyme was relatively stable a pH2.0~5.0 and at temperature below 4$0^{\circ}C$. Phenylmethane-sulfonyl fluoride and Ag+ inhibited the enzyme activity. This indicates that the enzyme is serine protease. Km value was 0.9$\times$10-4M and Vmax value was 5.93$\mu\textrm{g}$/min. This enzyme hydrolyzed casein more rapidly than bovine albumin.

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