Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization of Crude Oil Degradation by Klebsiella sp. KCL-1 Isolated from Sea Water

해양에서 분리된 Klebsiella sp. KCL-1에 의한 원유분해 특성의 규명

  • 차재영 (동아대학교 생명자원과학부) ;
  • 정선용 (전남대학교 공과대학 환경공학과) ;
  • 조용수 (동아대학교 생명자원과학부) ;
  • 최용락 (동아대학교 생명자원과학부) ;
  • 김범규 (경상대학교 유전공학연구소) ;
  • 이영춘 (동아대학교 생명자원과학부)
  • Published : 1999.12.01
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Abstract

Several bacterial strains utilizing crude oil as their sole carbon and energy sources were isolated from marine. One of the strains named KCL-1 showed the highest degradative activity for crude oil and the best growth rate. This strain was identified as a Klebsiella sp. based on the morphological, biochemical, and physiological characteristics. The optimum cultural conditions were as follows; $32^{\circ}C$~$37^{\circ}C$ for temperature and 7.0 for initial pH. Additionally, the optimal concentration of sodium chloride was 3.0%, indicating that this strain was derived from seawater. KCL-1 could use several kinds of n-alkane hydrocarbons from octadecane to hexacosane as a sole carbon source. The degradation of crude oil by KCL-1 was stimulated by addition of octadecane in the culture. The emulsifying activity by KCL-1 was highest after 3 days of cultivation under the condition of 3.0% sodium chloride, pH 7.0 and $37^{\circ}C$.

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