YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Induction of Cd-binding High Molecular Weight Protein [Cd-BP(II)] in Rat Tissues

흰쥐 조직에서의 카드뮴 결합 고분자량 단백질 [Cd-BP(II)]의 유도

  • Published : 1999.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

The study was carried out on the biochemical characters of Cd-BP(II) after isolation and purification of the protein from the liver of rat ip injection with Cd. A continued study has been doing whether Cd-BP(II) could be induced by Cd or by the other metals such as Zn and Cu. Antisera were made against the antigen of Cd-BP(II) from New Zealand white rabbits. We carried out g-globulin purification, then Ouchterlony test and gel immunodiffusion test. Cd-BP(II) was also found in normal tissues of rat. It was induced up to a considerable level by Cd, whose induced level was higher than that Cu or Zn treatment. The level of induction by Cu or Zn pretreatment plus Cd treatment was lower than that by single treatment of Cu or Zn. Such a result was presumably related to the Cd toxicity.

Keywords

References

  1. Toxicology v.70 Environmental pollutants and disease: A cell biological approach using chronic cadmium exposure in the animal model as a paradigm case Morselt, A. F. W.
  2. Metallothionein v.52 Chemistry and biochemistry of metallothionein. In Experientia Supplementum Kagi, J. J. R.;Kojima, Y
  3. Toxicology v.40 Consequences of cadmium toxicity in rat hepatoxytes: mitochondrial dysfunction and lipid peroxidation Muller, L
  4. Res. Commun. Chem. Pathol. Pharm v.74 Different distribution of cadmium in the liver supernatant between in vivo and in vitro Sunaga, H.;Yamane, Y.;Aoki, Y.;Suzuki, K. T.
  5. Anal. Biochem. v.72 A rapid and sensitive method for the quantitaino of microgram quantities of protein utilizing the principle of protein dye binding Bradford, M. M.
  6. Cadmium in the environemnt Winge,D.R.;Rajagolalan,K.V.
  7. Fed. Proc. v.32 Studies on the synthesis of cadmium binding protein using 109Cd, 115Cd, and 14C in liver and kidney Squibb, K. S.;Cousins, R. J.
  8. Toxicol. Appl. Pharmacol v.88 Protective role of renal metallothionein against Cd nephropathy in rats Min, K. S.;Hatta, A.;Onosaka, S.;Ohta, N.;Okada, Y.;Tanaka, K.
  9. Toxicol. Appl. Pharmacol v.86 Tissue distribution of cadimium and nephropathy after administration of cadmium in several chemical forms Min, K. S.;Kobayashi, K.;Onosaka, S.;Ohta, N.;Okada, Y.;Tanaka, K.
  10. Proc. Soc. Exp. Biol. Med. v.191 Degradation of zinc-metallothionein in monolayer cultures of rat hepatocytes Chen, M. L.;Failla, M. L.
  11. Toxicology v.36 The interaction of zinc and cadmium in the synthesis of hepatic metallo-thionein in rats Scheuhammer, A. M.;Onosaka, S.;Rodgers, K.;Cherian, M. G.
  12. Chem. Pharm. Bull v.25 Suppressive effect of zinc on the toxicity of mercury Yamane, Y.;Fukino, H.;Imagawa, M.
  13. J. Reprod. Fertil v.15 Mechanisms of zinc, cystein and selenium protection against cadmium-induced vascular injury to mouse testis Gunn, S. A.;Gould, T. C.;Anderson, W. A. D.
  14. Eisei Kagaku v.23 Substitution of zinc bound to metallothionein for cadmium in vitro and in vivo Tanaka K.;Onosaka, M. D.;Okahara, K.
  15. J .Nutr. v.117 Changes in dietary zinc result in specific alterations of metallothionein concentrations in newborn rat liver Gallant, K. R.;Cherian, M. G.
  16. Zinc proteins in rat liver. Second International symposium on trace element metabolism in animals, Madison, Wisc Bremner, I.;Davies, N. T.