YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
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Screening of Chitin Synthase II Inhibitors from Medicinal and Wild Plants

고등식물로부터 Chitin Synthase II 활성 저해물질의 탐색

  • 황의일 (생명공학연구소 식물보호소재 R.U.) ;
  • 이향복 (생명공학연구소 식물보호소재 R.U.) ;
  • 김성욱 (생명공학연구소 식물보호소재 R.U.)
  • Published : 1999.08.01
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Abstract

Chitin is an important structural component of fungal cell wall and is synthesized by chitin synthase I, II, and III. The chitin synthase II is an essential enzyme for the formation of primary septum in Saccharomyces cerevisiae. Therefore, specific inhibitors of this enzyme might block the formation of fungal cell wall and could be used as effective antifungal agents. To search chitin synthase IIinhibitors from natural products, 67 plants were extracted with methanol and examined for the inhibitory activities against chitin synthase II of S. cerevisiae by our cell free assay system. As a result, the extracts from 16 plants showed more than 70% inhibition at the concentration of $280{\;}\mu\textrm{g}/ml$. Of note, Laurus nobilis (81.4%), Lonicera maackii (81.5%), Berchemia berchemiaefolia (82.9%), Koelreuteria paniculata (87.9%), Chamaecyparis pisifera (86%) and Taxus cuspidata (83.9%) inhibited strogly the chitin synthase IIactivity.

Keywords

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