Screening of Alcohol Dehydrogenase Inhibitors from Natural Products

천연물로부터 알코올 탈수소효소 저해제 검색

  • 이현주 (이화여자대학교 약학대학·약학연구소) ;
  • 이강만 (이화여자대학교 약학대학·약학연구소)
  • Published : 1999.08.01

Abstract

Excessive or long term ingestion of alcohol may cause hepatitis, cirrhosis, hepatic tumor and so on. Aldehyde and active form of free oxygen that are metabolites of alcohol in liver are the cause of liver cell damage. The main system of alcohol metabolism is composed of alcohol dehydrogenase (ADH), aldehyde dehydrogenase (ALDH) and cytochrome P450. In connection with in vivo alcohol metabolism, more than one hundred natural products were screened for inhibition or activation of alcohol dehydrogenase. As a results, we found significant inhibition ($IC_50$) of ADH by methanolic extracts of Puerariae Radix ($61.2{\;}\mu\textrm{g}/ml$), Glycyrrhizae Radix ($105.0{\;}\mu\textrm{g}/ml$), Cinnamomi Ramulus ($7.0{\;}\mu\textrm{g}/ml$), Rhei Rhizoma ($36.7{\;}\mu\textrm{g}/ml$), Mori Cortex Radicis ($106.2{\;}\mu\textrm{g}/ml$), Chrysanthemi Flos ($112.2{\;}\mu\textrm{g}/ml$), Erycibes Caulis ($36.7{\;}\mu\textrm{g}/ml$), and Scutellariae Radix ($122.5{\;}\mu\textrm{g}/ml$)

Keywords

References

  1. Clin. Chim. Acta v.257 Ethanol metabolism, cirrhosis and alcoholism Lieber C. S.
  2. Semin. Liver. Dis v.13 no.2 Biochemical factors in alcoholic liver disease Lieber C. S.
  3. Update, Gastroenterology v.106 Alchol and the Liver Lieber C. S.
  4. J. Pharmacol. Exp. Ther v.264 no.2 Cytochrome P450 CYP 2E1 induction during chronic alcohol exposure occurs by a two-step mechanism associated with blood alcohol concentration in rats Ronis, M.J.;Huang, J.;Crouch, J.;Mercado, C.;Irby, D.;Valentine, C. R.;Lumpkin, C.K.;Ingelman-Sundberg, M.;Badgerm T. M.
  5. 현대생약학 생약학연구회
  6. 한약학 한약연구 소위원회
  7. 고금명방 권중록;박영애 (공역)
  8. 동양전통약물원색도감 김재길
  9. 한국의 식물자원 김태정
  10. Arch. Pharm. Res v.20 Ingibition of ethanol absortion by Rhodiola sachalinensis in rats Kim, M. H.;Park, C. K.
  11. Yakhak Hoeji v.41 The characteristics of I269S double mutant horse liver alcohol dehydrogenase Ryu, J. W.;Lee, K. M.
  12. Alcohol Clin. Exp. Res v.17 no.6 Biochemical studies of a new class of alcohol dehydrogenase inhibitors from Radix puerariae Keung, W. M.
  13. Experientia Supplementum v.71 Therapeutic lessons from traditional oriental medicine to contemporary occidental pharmacology Keung, W. M.;Vallee, B. L.
  14. Proc. Natl. Acad. Sci. USA 30 v.93 no.9 Potentiation of the bioavailability of daidzin by an extract of Radix puerariae Keung, W. M.;Lazo, O.Kunze, L.;Vallee, B.L.
  15. Alcohol Clin. Exp. Res v.20 no.4 Isoflavonoid compounds extracted from Pueraria lobata suppress alcohol preference in a pharmacogenetic rat model of alcoholism Lin, R. C.;Guthrie, S.;Xie, C. Y.;Mai, K.;Lee, D. Y.;Lumeng, L.;Li, T. K.
  16. Am. J. Clin. Nutr v.68 no.6 Effects of isoflavones on alcohol pharmacokinetics and alcohol-drinking behavior in rats Lin, R. C.;Li, T. K.
  17. Alcohol Clin. Exp. Res v.18 no.6 Daidzin, an antioxidant isoflavonoid, decreases blood alcohol levels and shortens sleep time induced by ethanol intoxication Xie, C. I.;Lin, R. C.;Antony, V.;Lumeng, L.;Li, T. K.;Mai, K.;Liu, C.;Wang, Q. D.;Zhao, Z. H.;Wang, G. F.