YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Studies on the Comparison of Scutellaria Radix Analyses by CE and HPLC

모세관전기영동장치 및 고속액체 크라마토그래피에 의한 황금의 성분 분석법 비교

  • 면노홍 (동덕여자대학교 약학대학) ;
  • 김효진 (서울특별시보건환경연구원)
  • Published : 1999.08.01
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Abstract

A simple, accurate and reproducible Capillary electrophoresis (CE) assay has been developed for the determination of baicalin, baicalein, wogonin and chrysin in Scutellaria baicalensis. Successful separation of these compounds has been obtained in 35 mM phosphate butter (pH 7.0) using a untreated fused silica capillary ($57{\;}cm{\times}75{\;}{\mutextrm{m}}$ i.d.) at $25^{\circ}C$ with the electric field of 19kV. Baicalin, baicalein wogonin and chrysin was separated and detected at 280 nm 13 min. The detection limits of CE were acceptable compared to HPLC. Reproducibilities of migration time and peak area were 0.66~1.11% (within-run), 2.18~3.38% (between-run) and 3.50~4.55% (within-run), 3.97~4.82%(between-run) at CE. The results indicate that CE could be a promising technique for quality and quantity control analysis of Scutellaria baicalensis as a validation method.

Keywords

References

  1. 대한약전6개정 대한보건공정서협회
  2. 신농본초경 v.2 손성연
  3. 중국본초도록 제2권 v.2 연문담
  4. 대한약전외 한양(생약)규격집 지형준;이상민
  5. 본초학 이상인;안덕균
  6. Yakugaku Zasshi v.99 On the revised structure of Skullcapflavone Ⅰ. a flavone compound in the roots of Scutellaria baicalensis George (wogon) Takido M.;Yasukawa K.;Matsuura S.;Iinuma M.
  7. Chem. Pharm. Bull v.32 Studies on Scutellaria baicalensis Ⅶ. Anti-arthritic and acti-inflammatory actions of methanolic extract and flavonoid compnents from Scutellaria Radix Kubo M.;Matsuda H.;Tanaka M.;Kimura Y.;Okuda H.;Higashino M.;Tani T.;Namba K.;Archi S.
  8. Shoyakugaku Zasshi v.42 no.3 Studies on the constituents of Scutellaria Species. (X) on the constituents of the leaves of Scutellaria baicalensis Georgi Miyaichi Y.;Inoto Y.;Saida H.;Tomimori T.
  9. Shoyakugaku Zasshi v.44 no.1 Studies on the constituents of Scutellaria Species.(ⅩⅡ) effects of buds picking and inflorescences picking on the growth and flavonoid content in the root of Scutellaria baicalensis Georgi Miyaichi Y.;Jin H.;Yamamoto M.;Tomimori T.;Mikage M.;Namba T.
  10. Acta Pharmaceutica Sinica v.26 no.5 Studies on the constituents of Scutellaria Species. (Ⅳ) HPLC of glucuronylfavonoids in Scutellaria Ikonnikovii Juz Wang Y. Q.;Matsuzaki K.;Takahashi K.;Okuyama T.;Shibata S.
  11. Chem. Pharm. Bull v.36 no.8 Studies on the constituents of Scutellaria Species. (Ⅰ) The flavonoid glucuronides of "Bo Ye Huang Chin." Wang Y. Q.;Keiichi M.;Kunio T.;Toru O.;Shoji-S
  12. Phytochemistry v.20 no.10 Flavone Di-C-Glycosides from Scutellaria baicalensis Takagi S.;Yamaki M.;Inoue K.
  13. 생약회지 v.23 Flavonoid components in plants of the genus Scutellaria 윤혜숙
  14. Phytochemistry v.35 no.2 Four flavonoids from Scutellaria baicalensis Zang Y.Y.;Guo Y.Z.;Onda M.;Hashimoto K.;Ikeya Y.;Okada M.;Mauno M.
  15. Yakugaku Zasshi v.95 Stueies on the constituents in the water extracts of crude drugs Ⅰ. roots of Scutellaria baicalensis George (wogon) Takato M.;Aimi M.;Takuhashi S.;Yamanouchi S.;Torill H.;Dohi S.
  16. Yakugaku Zasshi v.105 no.2 Studies on the constituents of Scutellaria Species Ⅵ. On the flavonoid constituents of the root of Scutellaria baicalensis Georgi (5) Quantitative analysis of flavonoids in Scutellaria Roots by HPLC Tomomori T.;Jim H.;Miyaichi Y.;Toyofuku S.;Namba T.
  17. Zhongcaoyao v.21 no.12 Scutellaria Species Ⅵ. Comparison of baicalin content in eight kinds of Scutellaria Species by RP-HPLC and determination of their blood coagu-lation and fibrinolytic acticity Wang Y.;Lu L.;LiD.;Yang M.;Takahashi K.;Narui T.;Wang Z.;Okuyama T.;Shiabata S.
  18. Shoyakugaku Zasshi v.40 no.1 Determination of flavonoids of Scutellaria Radix in pharmaceutical preparations by ion-pair HPLC Sagara K.;Ito Y.;Oshima T.;Murayama H.;Itokawa H.
  19. Shoykugaku Zasshi v.45 no.3 Micellar electrokinetic chromatography for the analysis of crude drigs (Ⅰ). Determination of glycyrrhizin in oriental phamaceutical preperations Iwagami S.;Sawaba Y.;Nakagawa T.
  20. J. Korean Chemical Society v.41 no.2 모세관 전기 이동법에 의한 생약제제중 베르베린, 계피산 및 글리실리진의 정량 강성호;정화진;윤형중;정두수
  21. 약학회지 v.41 no.5 모세관 전기영동 분석법의 복합 약물제제의 품질관리 분석에 응용을 위한 연구 허유정;이공주
  22. 식품의약품안전본부연보 v.1 no.207 생약제제의 약제학적 연구(Ⅹ)-황금 및 그 함유제제 처방에 따른 성분 추출량에 관한 연구 강신복;최보경;최명희;김영림;오미현;지선경;원봉필;김영중