Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
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Effects of Wounding and Jasmonic Acid on Polyphenol Oxidase in Tomato Seedlings

토마토 유식물의 Polyphenol Oxidase에 미치는 상해 및 Jasmonic Acid의 영향

  • 진선영 (부산대학교 자연과학대학 생물학과) ;
  • 홍정희 (부산대학교 자연과학대학 생물학과)
  • Published : 1999.12.01
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Abstract

The effects of wounding and jasmonic acid(JA) on polyphenol oxidase(PPO) in tomato(Lycopersicon esculentum Mill.) seedlings were investigated. PPO was strongly induced by wounding or JA, and the response was also shown to be systemically induced by wounding. Mechanical wounding in cotyledon or leaf produced a signal that caused the concentration of PPO to increase in the unwounded cotyledon, in the first leaves but not in the second leaves. Severity of wounding and light intensity also affected wound induced change in PPO activity, JA showed a stimulatory effect on the loss of chlorophyll and the rapid increase in PPO activity. The PPO was clearly more active in the wounded leaves than in controls. The potency and specificity of the JA indicate a close relationship between JA and wound-induced changes in PPO in tomato species. JA and abscisic acid(ABA) acted similarly on both unwounded and wounded leaves, but the amount of PPO in the wounded leaves was always more than the respective controls. The highest increase in PPO activity occurred in woundand JA-induced leaves of seedlings kept under bright lighting. Benzyladenine(BA) completely abolished JA- and ABA-induced PPO activity. The results suggest that JA-induced PPO activity is due to de novo PPO synthesis. Histochemical tests for PPO in stems of wound- and JA -treated tomato plants indicate that PPO was localized primarily, in the. outer .cortex . and xylem parenchyma. It is concluded that exogenously applied JA acts as stress agents and PPO may be a component of the inducible anti-hervivore defense response.

Keywords

References

  1. Proc. Natl. Acad. Sci. U.S.A. v.92 Systemin activates synthesis of wound inducible tomato leaf polyphenol oxidase via the octadecanoid defense signaling pathway Constabel, C.P.;D.R. Bergey;C.A. Ryan
  2. In Plant-Insect Interactions v.V Baldwin, I.T.;E.A. Bernays(ed.)
  3. Phytochemical Induction by Herbivores Tallamy, D.W.;M.J. Raupp(ed)
  4. In Naturally Occurring Pest Bioregulators Duffey, S.S.;G.W. Felton;P.A. Hedin(ed.)
  5. Phytochem. v.31 Effects of wounding on glucosinolates in the cotyledons of oilseed rape and mustard Bodnaryk, R.P.
  6. Plant Physiol. v.98 Regulation of expression of proteinase inhibitor genes by methyl jasmonate and jasmonic acid Farmer, E.E.;R. Johnson;C.A. Ryan
  7. Biochem. Biophys. Acta v.1165 Oxylipin pathway to jasmonates : Biochemistry and biological significance Hamberg, M.;H.W. Gardner
  8. J. Plant Growth Regul. v.3 Occurrence of the plant growth regulator jasmonic acid in plants Mayer, A.;O. Niersch;C. Buttner;W. Dathe;G. Sembdner
  9. Annu. Rev. Plant Physiol. Plant Mol. Bio. v.44 The biochemistry and physiological and molecular actions of jasmonates Sembdner, G.;B. Parthier
  10. Plant Cell Physiol. v.35 Jasmonic acid inhibits the IAA-induced elongation of oat coleoptile segments : A possible mechanism involving the metabolism of cell wall polysaccharides Ueda, J.;K. Miyamoto;M. Aoki
  11. Physiol. Plant. v.54 Inhibition of cytokinin induced plant growth by jasmonic acid and its methyl ester Ueda, J.;J. Kato
  12. Plant Physiol. v.99 Methyl jasmonate, calcium, and leaf senescence in rice Chou, C.M.;C.H. Kao
  13. Plant Physiol. v.84 Stimulation of adventitious rooting in mung bean seedlings by jasmonic acid and 12-oxo-phytodienoid acid Zimmerman, D.C.;J.S. Bush;J.A. Kuc.
  14. Phytochem. v.30 Potato tuberinducing activities of jasmonic acid and related compounds Koda, Y.;Y. Kikuta;H. Tazaki;Y. Tsujino;S. Sakamura;T. Yoshihara
  15. Plant, Cell and Environment v.20 Stomatal responses to jasmonic acid, linolenic acid and abscisic acid in wild-type and ABA-deficient tomato plants Herde, O.;H. Pena Cortes;L. Willmitzer;J. Fisahn
  16. Plant Physiol. v.99 Jasmonate, genes and fragrant signals Staswick, P.
  17. J. Plant Physiol. v.143 Methyl jasmonate induces the accumulation of putrescine but not proline in detached rice leaves Chen, C.T.;C.M. Chou;C.H. Kao
  18. Bot. Acta v.104 Jasmonates, new regulators of plant growth and development : Many facts and few hypotheses on their actions Parthier, B.
  19. Proc. Natl. Acad. sci. U.S.A. v.87 Interplant communication : Airborne methyl jasmonate induces synthesis of proteinase inhibitors in plant leaves Farmer, E.E.;C.A. Ryan
  20. Proc. Natl. Acad. Sci. U.S.A. v.89 Jasmonic acid is a signal transducer in elicitor-induced plant cell culture Gundlach, H.;M. Muller;T.M. Kutchan;M.H. Zenk
  21. Planta v.197 Accumulation of jasmonate, abscisic acid, specific transcripts and proteins in osmotically stressed barley leaf segments Lehmann, J.R. Atzorn;C. Bruckner;S. Reinbothe;J. Leopold;C. Wasternack;B. Parthier
  22. Proc. Natl. Sci. U.S.A. v.92 The octadecanoid pathway : Signal molecules for the regulation of secondary pathway Blechert, S.;W. Brodschelm;S. Holder;L. Kammerer;T.M. Kutchan;M.J. Mueller;Z Q. Xia;M.H. Zenk
  23. The Plant Cell v.4 Octadecanoid precursors of jasmonic acid activate the synthesis of wound inducible proteinase inhibitors Farmer, E.E.;C.A. Ryan
  24. J. Chem. Ecol. v.15 Activation of plant foliar oxidases by insect feeding reduces nutritive quality foliage for noctuid hervivores Felton, G.;K. Donato;R. Del Vecchio;S. Duffey
  25. Phytochem. v.30 A limited survey of the phytogenetic distribution of the phytogenetic distribution of polyphenol oxidase Sherman, T.D.;K.C. Vaughn;S.O. Duke
  26. J. Biol. Chem. v.265 Sodium dodecyl sulfate activation of plant polyphenol oxidase Moore, B.M.;W.H. Flurkey
  27. Phytochem. v.30 A limited survey of the phylogenetic distribution of polyphenol oxidase Sherman, T.D.;K.C. Vaughn;S.O. Duke
  28. Plant Physiol. v.24 Copper enzymes in isolated chloroplast : Polyphenol oxidase in Beta vulgaris Arnon, D.
  29. Can. J. Bot. v.59 Localization of phenols and polyphenol oxidase in 'Jewel' sweet potatoes Schadel, W.E.;W.M. Walter
  30. Phytochem. v.47 A survey of wound- and methyl jasmonate-induced leaf polyphenol oxidase in crop plants Constabel, C.P.;C.A. Ryan
  31. Physiol. Plant. v.90 Presence and role of jasmonate in apple embryo Ranjan, R.;O. Miersch;G. Sembdner;S. Lewak
  32. Plant Physiol. v.98 Changes in the polypeptide patterns of barley seedlings exposed to jasmonic acid and salinity Maslenkova, L.T.;T.S. Miteva;L.P. Popova
  33. Plant Physiol. v.82 Effects of wounding on cytokinin activity in cucumber cotyledons Crane, K.E.;C.W. Ross
  34. Plant Physiol. v.70 Tissue distribution of chlorogenic acid and of enzymes involved in its metabolism in leaves of Sorghum bicolor Kojima, M.;E.E. Com
  35. Phytomorph. v.45 Histochemical localization of oxidase in Parthenium argentatum Jayabalan, M.;J.J. Shah;K. Rajarathinam;S. Veerasamy
  36. Jasmonates: Metabolism, biological activities, and modes of action in senescence and stress responses, In Propress in Plant Ggrowth Regulation Parthier, B.;C. Bruckner;W. Dathe;B. Hause;G. Herrmann;H.D. Knofel;H.M. Kramell;R. Kramell;J. Lehmann;O. Miersch;S. Reinbothe;G. Sembdner;C. Wasternack;U. Zur Nieden;C.M. Karssen;L.C. van Loon;D. Vreugdenhil(ed)