BMB Reports
- Volume 32 Issue 1
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- Pages.86-91
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- 1999
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- 1976-670X(eISSN)
Optimal Production Conditions of Streptomyces griseus Trypsin (SGT) in Streptomyces lividans
- Koo, Bon-Joon (R&D Center, TS Corporation) ;
- Kim, Joung-Mee (Department of Biological Science, Myongji University) ;
- Byun, Si-Myong (Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), and Research Center for New Bio-Materials in Agriculture (RCNBMA)) ;
- Hong, Soon-Kwang (Department of Biological Science, Myongji University)
- Published : 1999.01.31
Abstract
The sprT gene encoding Streptomyces griseus trypsin (SGT) was introduced into Streptomyces lividans TK24 and Streptomyces lividans 1326 to study which strain would be better to overexpress the extracellular proteinase. Various media with different compositions were also used to maximize the productivity of SGT in heterologous hosts. The SGT productivity was best when the transformants of S. lividans TK24 and 1326 were cultivated in R2YE medium, and their relative trypsin activity of the culture broth measured with an artificial chromogenic substrate, N-