Histological Effects on the Liver and Kidney of Rats after Oral Administration of Radix Stemonae

백부근(百部根)이 흰쥐의 간(肝) 및 신장(腎臟)에 미치는 조직학적(組織學的) 연구(硏究)

  • Choi, Hae-Yun (Dept. of Internal Medicine, College of Oriental Medicine, Kyungsan University) ;
  • Kim, Jong-Dae (Dept. of Internal Medicine, College of Oriental Medicine, Kyungsan University)
  • 최해윤 (경산대학교 한의과대학 폐계내과학교실) ;
  • 김종대 (경산대학교 한의과대학 폐계내과학교실)
  • Published : 1999.09.30

Abstract

Since Radix Stemonae was recorded hypothennal and a little toxic in the 'Myngyubelrok (名醫別錄)', it has been recorded as having the same nature in many herbal books. However, the security of Radix Stemonae when used to treat respiratory disease over a long term has not been studied until now. Therefore, the objective of this study is to determine the effects of Radix Stemonae on the main organs if Radix Stemonae is administrated over a long term. In order to investigate the histological changes of the liver and kidneys of rats after oral administration of Radix Stemonae extract, the experimental rats were subdivided into control, 1, 3, 5, 7, 21, 28 and 35 days after administration groups, and 10 rats per group were used in this study. The control group was sufficiently supplied with water and solid forage. The other groups were administrated the reagent at 5mg/kg once a day by oral injection. Several times each day, the experimental groups were carefully observed for any changes of general condition, toxic symptoms, activity, appearance and the number of dead rats. The experimental groups were weighed and narcotized. For the histological observation, the tissues of liver and kidneys of the experimental groups were collected, stained by hematoxylin-eosin stain, and evaluated by observing the changes of gross appearance and by observing microscopic findings. 1. This drug, during the experimental term, did not induce any toxicological effect in mortality, abnormal symptoms or changes of body weight except for the 1 day after administration groups whose body weights were decreased, compared to the control group. 2. No gross changes of the liver and kidneys were observed in this study. 3. No histological changes of the liver were detected in 1 day after administration groups. However, dilation of the central vein was observed in 3, 5 and 7 days after administration groups and chronic passive congestion of the liver was demonstrated in the 21 days after administration groups. In the 28 and 35 days after administration groups, a centrolobular disposition of fatty tissue (adipose cell) was observed. 4. No histological changes of the kidneys were observed in this study. It is evaluated that if Radix Stemonae is administrated for a long term, it induces toxicity in the liver. So, to examine the toxicity of Radix Stemonae on the liver and kidney, it is necessary that the studies of biochemistry and electron microscopic findings about Radix Stemonae be systematically performed.

Keywords

References

  1. 本草詩箋 朱東樵
  2. 增補本草備要 汪인庵
  3. 本草綱目(上) 李時珍
  4. 本草求眞 黃宮繡
  5. 本草從新 吳儀洛
  6. 東醫寶鑑 許浚
  7. 原色臨床本草學 辛民敎
  8. 本草學 李尙仁
  9. 申氏本草學 申佶求
  10. 本草綱目通釋 陳貴廷
  11. 毒性本草 楊倉良
  12. 全國中草藥匯編(上冊) 全國中草藥匯編編寫組
  13. 韓藥포製學 安德均;金頀哲
  14. 中藥大辭典 江蘇新醫學院(編)
  15. 中藥志 中國醫學科學院藥物硏究所(等)(篇)
  16. 中藥學 顔正華
  17. 實用臨證中藥指南 林通國
  18. 臨床中藥手冊 賀興東;鐘공生
  19. 中華藥海 염先德
  20. Applied veterinary histology. Banks, W.J.
  21. Thomson's special veterinary pathology. Carlton, W.W.
  22. Histology a text and atlas. Ross, M.H.;Romrell, L.J.;Kaye, G.I.
  23. Laboratory animal medicine Fox, J.G.;Cohen, B.J.;Loew, F.M.
  24. Jpn. J. Pharmacology v.20 Effects of adrenergic blocking agents on gastric secretion and stress-induced gastric ulcer in rats Okabe, S.;Saziki, R.;Takagi, K.
  25. 그림으로 설명한 병리학 이중달
  26. 그림으로 설명한 병리학 이중달
  27. 實驗動物の臨床生化學デ一タ一病理組織像との關蓮一 長瀨すみ;田中壽子
  28. 名醫別錄 陶弘景
  29. 重修政和經史證類備用本草 唐愼徵