Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Comparative Study on the Toxic Mechanism of Oxidant-Induced Neurotoxicity and Protective Effects of Several Herb Extracts as a Nerve Growth Factor in Spinal Motor Neurons Damaged by Oxygen Radicals

신경성장인자(神經成長因子)로서의 약류별(藥類別) 한약제(韓藥劑)가 척수(脊髓) 운동신경세포(運動神經細胞)의 손상(損傷)에 미치는 효능(效能) 및 기전(機轉)에 관(關)한 비교(比較) 연구(硏究)

  • Park Seung-Taeck (Dept. of Anatomy, School of Medicine, Wonkwang University) ;
  • Yoon Hyang-Suk (Depart. of Pediatrics, School of Medicine, Wonkwang University) ;
  • Hyoung Keon-Young (Depart. of Internal Medicine, School of Medicine, Wonkwang University) ;
  • Cho Chung-Gu (Depart. of Internal Medicine, School of Medicine, Wonkwang University) ;
  • Lee Kang-Chang (Depart. of Anesthesiology, School of Medicine, Wonkwang University) ;
  • Kim Won-Shin (Depart. of Life Science, College of Natural Science, Wonkwang University) ;
  • Kim Hyung-Min (Depart. of Life Science, College of Parmacy, Wonkwang University) ;
  • Jeon Byung-Hoon (Depart. of Pathology, College of Oriental Medicine, Wonkwang University) ;
  • Yun Young-Gap (Depart. of Prescription, College of Oriental Medicine, Wonkwang University)
  • 박승택 (원광대학교 의과대학 해부학교실) ;
  • 윤향석 (원광대학교 의과대학 소아과학교실) ;
  • 형근영 (원광대학교 의과대학 내과학교실) ;
  • 조정구 (원광대학교 의과대학 내과학교실) ;
  • 이강창 (원광대학교 의과대학 마취과학교실) ;
  • 김원신 (원광대학교 자연과학대학 생명과학부) ;
  • 김형민 (원광대학교 자연과학대학 한약학과) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실) ;
  • 윤용갑 (원광대학교 한의과대학 방제학교실)
  • Published : 1999.11.25
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Abstract

In order to eludidate the mechanism of oxidative stress in cultured spinal motor neurons damaged by oxygen free radicals, cytoxicity was assesed by MTT assay and NR assay after spinal motor neurons from mouse were cultured in media containing various concentrations of xanthine oxidase(XO) and hypoxanthine(HX) for 3 hours. In addition, neuroprotective effects of several herb extracts on oxidant-induced neurotoxicity were examined in these cultures, compared with nerve growth factors such as basic fibroblast growth factor(bFGF). XO/HX decreased cell viability in dose- and time dependent manners on cultured mouse spinal motor neurons, and MTT50 and NR50 values were measured at 20mU/ml XO and 0.1mM HX for 3 hours in these cultures. bFGF significantlt increased cell viability. In neuroprotective of herb extracts, Epimedium Koreanum Nakai(EK) and Alpinia oxphylla Mig(IJI) was very effective in the prevention of the neurotoxicity induced by XO/HX in cultured mouse spinal motor neurons. From the above results, it is suggested that XO/HX shows toxic effect in cultured mouse spinal motor neurons and selective herb extracts such as Epimedium Koreanum Nakai(EK) and Alpinia oxphylla Mig(IJI) were very effective in the increase of cell viability against the neurotoxicity induced by oxygen radicals in these cultures.

Keywords

References

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