Korean Journal of Food Science and Technology (한국식품과학회지)
- Volume 31 Issue 6
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- Pages.1628-1634
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- 1999
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- 0367-6293(pISSN)
Specific Detection of Listeria monocytogenes in Foods by a Polymerase Chain Reaction
PCR에 의한 식품으로부터 Listeria monocytogenes의 특이적 검출
- Shin, Soon-Young (Korea Food Research Institute) ;
- Koo, Young-Jo (Korea Food Research Institute) ;
- Kim, Wang-June (Korea Food Research Institute)
- Published : 1999.12.31
Abstract
The polymerase chain reaction (PCR) for the sensitive and specific detection of Listeria monocytogenes was employed by using LM 1 and LM 2 primers which were based on the listeriolysin O gene. The direct use of cell suspension as DNA template, without DNA extraction or lysis step, was suitable and specific enough to detect L. monocytogenes at the level of
Listeria monocytogenes의 식품 속에서 신속하고 특이적인 검출을 위하여, listeriolysin O gene에 의한 primer, LM 1과 LM 2를 선택하여 PCR을 수행하였다. L. monocytogenes의 DNA 추출이나 cell lysis 없이 intact whole cell을 직접 이용하여 PCR을 하였으며