The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지

Anti-Varicella Zoster Virus Activity of Water Soluble Components of Elfvingia applanata Alone and in Combinations with Interferons

잔나비걸상 수용성성분의 항-Varicella Zoster Virus 작용과 Interferon과의 병용효과

  • Published : 1999.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

To search for less toxic antiviral agents from Basidiomycetes, the water soluble components (=EA), were isolated from the carpophores of Elfvingia applanata (Pers.) Karst. Anti-varicella zoster virus (Oka strain; anti-VZV/Oka) activity of EA was examined in MRC-5 cells by plaque reduction assay in vitro. And the combined antiviral effects of EA with interferon (IFN) alpha or IFN gamma were examined on the multiplication of VZV/Oka. EA exhibited a dose-dependent reduction in the plaque formation of VZV/Oka with 50% effective concentration $(EC_{50})$ of $464.14\;{\mu}g/ml$. The results of the combination assay were evaluated by the combination index (CI) that was calculated by the multiple drug effect analysis. The combination of EA with IFN alpha showed partially synergistic or additive effects with CI values of $0.83{\sim}1.09$ for 50%, 70%, 90% effective levels, and those with IFN gamma showed antagonism with CI values of $1.20{\sim}1.24$.

잔나비걸상 Elfvingia applananta 자실체의 수용성성분 EA의 varicella zoster virus(Oka strain; VZV/oka)에 대한 항바이러스효과를 plaque reduction assay에 따라 실험한 결과 EA는 용량의존적으로 plaque 형성을 억제하였으며 $EC_{50}$는 $464.14\;{\mu}g/ml$이었다. EA와 단백질성 항바이러스제인 interferon(IFN) alpha 및 IFN gamma와의 병용시험 결과, 단독처리시와 병용처리시에 m(slope) value가 서로 유사한 값을 보였으므로 이들은 상호 배타적인 약물(mutually exclusive drug)임을 알 수 있었고, IFN alpha와의 병용시 combination index(CI)는 f(a)가 0.50에서 0.90인 유효농도 범위내에서 $0.83{\sim}1.09$의 값을 나타내어 부분적 상승 또는 상가효과를 보였으며, IFN gamma와의 병용시에는 $1.20{\sim}1.24$를 나타내어 길항효과를 보였으므로, IFN alpha와의 병용이 IFN gamma와의 병용보다 더 우수한 병용효과를 나타내었다.

Keywords

References

  1. Adv. Enzyme Regul. v.22 Quantitative analysis of dose-effect relationships; The combined effects of multiple drugs or enzyme inhibitors Chou, T.C.;Talalay, P.
  2. J. Antimicrob. Chemother. v.18 no.Sup. B General aspects of virus resistance with special reference to herpes simplex virus Coen, E. M.
  3. Arch. Virology. v.51 An improved plaque-assay for varicella virus Hondo, R.;Shibuta, H.;Matsumoto, M.
  4. Acta Microbiol. Pol. v.28 Fungal nucleic acids as interferon inducers Kandefer, S.M.;Kawecki, Z.;Guz, M.
  5. Kor. J. Mycol. v.8 Studies on the antineoplastic components of Korean Basidiomycetes Kim, B.K.;Chung, H.S.;Chung, K.S.;Yang, M.S.
  6. Kor. J. Pharmacogn. v.25 Effects of the antitumor component, F-D-P, isolated from Elfvingia applanata on the immune response Kim, Y.S.;Ryu, K.H.;Mo, Y.K.;Lee, C.K.;Han, S.S.
  7. Yakhak Hoeiji v.38 Antimicrobial activity of Elfvingia applanata extract alone and in combination with some antibiotics Kim, Y.S.;Ryu, K.H.;Lee, C.K.;Han, S.S.
  8. Yakhak Hoeji v.38 Effect of Elfvingia applanata extract on the acute toxicity in mice Kim, Y.S.;Kang, J.K.;Lee, C.K.;Han, S.S.
  9. Il Farmaco v.48 no.7 Antiherpes virus agents: A review Lapucci, A.;Macchia, M.;Parkin, A.
  10. Shizuoka Daigaku Nogakubu Kenkyu Hokoku v.31 Host-mediated antitumor polysaccharides. III. Fractionation, chemical structure, and antitumor activity of water-soluble homoglucans isolated from kofukisarunokoshikake, the fruit body of Ganoderma applanatum Mizuno, T.;Hayashi, K.;Arakawa, M.;Shinkai, K.;Shimizu, M.;Tanaka, M.
  11. Phytochemistry v.28 Bitter triterpenoids from the fungus Ganoderma applanatum Nishitoba, T.;Goto, S.;Sato, H.;Sakamura, S.
  12. Coll Czech Chem. Commun. v.45 Triterpenes LXIII. Triterpenes and steroids from Ganoderma applanatum Protiva, J.;Skorkovska, H.;Urban, J.;Vystrcil, A.
  13. Kor. J. Pharmacogn. v.30 no.1 Antiviral activity of water soluble substance from Elfvingia applanata Rym, K.H.;Eo, S.K.;Kim, Y.S.;Lee, C.K.;Han, S.S.
  14. Combined therapeutic modalities for viruses-rationale and clinical potential. Molecular mechanism of chemotherapeutic synergism, potentiation and antagonism Schinazi, R.F.;Chou, T.C.(ed.);Rideout, D.C.(ed.)
  15. Cancer Res. v.48 Evaluation of a soluble tetrazolium/ formazan assay for cell growth and drug sensitivity in cutlure using human and other tumor cell lines Scubiero, D.A.;Shoemaker, R.H.;Paull, K.D.;Monks, A.;Tierney, S.;Nofziger, T.H.;Currens, M.J.;Seniff, D.;Boyd, M.R.
  16. Antiviral Chem. Chemother. v.3 Synergistic inhibition of human immunodeficiency virus type I in vitro by interferon alpha and coumermycin A1 Tachedjian, G.;Tyssen, D.;Jardine, D.;Locarnini, S.;Birch, C.
  17. Carbohydr. Res. v.115 Isolation and characterization of antitumor active ${\alpha}-D-glucans$ from the fruit bodies of Ganoderma applanatum Usui, T.;Iwasaki, Y.;Mizuno, T.;Tanaka, M.;Shinkai, K.;Arakawa, M.