The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지

The Specific Probes Confirming the Genomic DNA of Tricholoma matsutake in Korea

송이의 Genomic DNA에 특이적인 Probe

  • Lee, Sang-Sun (Korea National University of Education) ;
  • Hong, Sung-Woon (Korea National University of Education) ;
  • Chung, Hung-Chae (Korea National University of Education) ;
  • Sung, Chang-Kun (Department of Food Science and Technology, College of Agriculture, Chung-Nam National University) ;
  • Kim, Jae-Hun (Department of Food Science and Technology, College of Agriculture, Chung-Nam National University) ;
  • Ka, Kang-Hyeon (Division of Wood Chemistry & Microbiology, Forestry Research Institute) ;
  • Kim, Hyun-Joong (Division of Wood Chemistry & Microbiology, Forestry Research Institute)
  • 이상선 (한국교원대학교 생물교육학과) ;
  • 홍성운 (한국교원대학교 생물교육학과) ;
  • 정흥채 (한국교원대학교 생물교육학과) ;
  • 성창근 (충남대학교 농과대학 식품공학과) ;
  • 김재훈 (충남대학교 농과대학 식품공학과) ;
  • 가강현 (임업연구원 화학미생물과) ;
  • 김현중 (임업연구원 화학미생물과)
  • Published : 1999.02.28
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Abstract

The specific DNA band appeared in PCR-RAPD analysis using OPO-2 primer was a very important for the researching Korean pine-mushrooms, Tricholoma matsutake. This DNA band, sequenced to be the 770 base pairs, existed as only a single copy in the whole genomic DNA's of Korean pine-mushrooms. However, this band was not presenting from the PCR-RAPD bands of other ectomycorrhyzal fungi reacted with the OPO-2 primer or the dot blots. Also, this DNA sequence was not matched with those of the other genes known by NCBI and had low homology together with sequence of other proteins compared. Those results suggested that the specific DNA band can be used as probe for identification of T. matsutake and might be related to the informations rather than the gene for the proteins with analysis of protein sequence translated from the DNA sequence.

우리나라에서 자생하는 송이버섯의 구분 방법으로 제조된 OPO-2 primer을 사용할 때 송이에게만 특이적인 band가 나타났다. 그리고 제한효소 처리와 dot blot의 결과에서 이들 특이적인 DNA절편은 송이 genomic DNA에서 한개의 copy만 존재하는 것으로 나타났으며, 다른 외생균근 버섯의 DNA에서는 발견되지 않았다. 또한, 이들 DNA 절편의 염기서열을 분석한 결과 770bps로 나타났다. 이러한 유전자의 정보를 이용한 NCBI Blast 염기서열 분석에서 높은 상동성을 갖는 유전자는 발견할 수가 없었고, 단백질서열 분석에서도 거의 동일하게 나타났다. 따라서 translation통한 아미노산 서얼분석에서 이들 DNA절편의 유전자는 단백질에 관한 유전자이라기 보다는 다른 정보와 관련된 유전자로 생각되어진다.

Keywords

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