The Korean Journal of Physiology and Pharmacology
- 제3권2호
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- Pages.127-135
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- 1999
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- 1226-4512(pISSN)
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- 2093-3827(eISSN)
Effects of Dexamethasone and DHEA on the Responses of Rat Cerebral Cortical Astrocytes to Lipopolysaccharide and Antimycin A
- Choi, Sang-Hyun (Department of Pharmacology, Korea University College of Medicine) ;
- Kim, Hyung-Gun (Department of Pharmacology, Korea University College of Medicine) ;
- Kim, Chang-Keun (Department of Pharmacology, Korea University College of Medicine) ;
- Park, Nan-Hyang (Department of Pharmacology, Korea University College of Medicine) ;
- Choi, Dong-Hee (Department of Pharmacology, Korea University College of Medicine) ;
- Shim, In-Sop (Department of Pharmacology, Korea University College of Medicine) ;
- Chun, Boe-Gwun (Department of Pharmacology, Korea University College of Medicine)
- 발행 : 1999.04.21
초록
As part of a study on the effects of dexamethasone and dehydroepiandrosterone (DHEA) on the biological roles of astrocytes in brain injury, this study evaluated the effects of dexamethasone and DHEA on the responses of primary cultured rat cortical astrocytes to lipopolysaccharide (LPS) and antimycin A. Dexamethasone decreased spontaneous release of LDH from astrocytes, and the dexamethasone effect was inhibited by DHEA. However, the inhibitory effect of DHEA on the dexamethasone-induced decrease of LDH release was not shown in astrocytes treated with LPS, and antimycin A-induced LDH release was not affected by dexamethasone or DHEA. Unlike dexamethasone, DHEA increased MTT value of astrocytes and also attenuated the antimycin A-induced decrease of MTT value. Glutamine synthetase activity of astrocytes was not affected by DHEA or LPS but increased by dexamethasone, and the dexamethasone- dependent increase was attenuated by DHEA. However, antimycin A markedly decreased glutamine synthetase activity, and the antimycin A effect was not affected by dexamethasone or DHEA. Basal release of
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