The Korean Journal of Physiology and Pharmacology
- 제3권1호
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- Pages.101-117
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- 1999
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- 1226-4512(pISSN)
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- 2093-3827(eISSN)
The Effects of Sera from Amyotrophic Lateral Sclerosis Patients on Neuromuscular Transmission and Calcium Channels in Mice
- Yan, Hai-Dun (Department of Physiology and Biophysics, Seoul National University College of Medicine) ;
- Kim, Ji-Mok (Department of Physiology and Biophysics, Seoul National University College of Medicine) ;
- Jung, Sung-Jun (Department of Physiology, Dankook university college of medicine) ;
- Kim, Jun (Department of Physiology and Biophysics, Seoul National University College of Medicine)
- 발행 : 1999.02.21
초록
Amyotrophic lateral sclerosis (ALS) is a degenerative neuromuscular disease of unknown etiology in which the upper and lower motor neurons are progressively destroyed. Recent evidences support the role of autoimmune mechanisms in the pathogenesis of ALS. This study investigated the effects of sera from ALS patients on neuromuscular transmission in phrenic nerve-hemidiaphragm preparations and on calcium currents of single isolated dorsal root ganglion (DRG) cells in mice. Mice were injected with either control sera from healthy adults or ALS sera from 18 patients with ALS of sporadic form, for three days. Miniature end plate potential (MEPP) and nerve-evoked end plate potential (EPP) were measured using intracellular recording technique and the quantal content was determined. Single isolated DRG cells were voltage-clamped with the whole-cell configuration and membrane currents were recorded. Sera from 14 of 18 ALS patients caused a significant increase in MEPP frequency in normal Ringer's solution
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