KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Isolation and Characteristics of Denitrifying Pseudomonas CW4

탈질균 Pseudomonas CW4의 분리 및 특성

  • Published : 1999.10.01
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Abstract

Ten denitrifying bacteria, which were identified as Pseudomonas sp., were isolated from Winogradsky columns. The most effective denitrifying bacterium was named as Pseudomonas CW4, which was cultivated at anoxic condition. The optimal growth temperature and pH were 3$0^{\circ}C$ and 6-8, respectively. The effect of carbon concentration and agitator speed on the rate of denitrification were very low. 100% of NO$_3$-N was removed after 15 hrs when initial concentration of NO$_3$-N was 142.5 mg/L.

Winogradsky column을 이용하여 10개의 탈질 박테리아를 분리하였다. 그 중 가장 성능이 우수한 탈질 박테리아를 Pseudomonas CW4로 명하였다. Pseudomonas CW4는 무산소 조건에서 배양하였고, Pseudomonas CW4의 최적 생육조건을 온도, pH. 교반속도, 탄소원 농도 및 질산성질소 농도에 변화를 주어 측정하였다. 최적 생육 온도 3$0^{\circ}C$, 최적 pH 범위는 6~8이었다. 교반속도와 탄소원 농도에 대한 영향은 아주 작았다. 그리고 질산성 질소의 초기농도 142.5 mg/L 에서 15 시간만에 100% 탈질을 나타내었다.

Keywords

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