KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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The Study on the Effective Expression Strategy for Recombinant Protein Production with Pichia pastoris and Hansenula polymorpha

Hansenula polymorpha와 Pichia pastoris의 비교를 통한 회분식 배양에서의 효과적인 재조합단백질 발현방법에 관한 연구

  • Published : 1999.08.01
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Abstract

As host for the production of eucaryotic heterologous proteins, methylotrophic yeast Pichia pastoris and Hansenula polymorpha are the most highly developed of a small group of alternative yeast species chosen for their perceived advantages. This paper describes the method to enhance the recombinant protein productivity with P. pastoris and H. Plymorpha. In these experiments, the effects of methanol induction timing, induction method, pH, culture temperature and kinds of nitrogen sources on foreign protein production were tested with P. pastoris and compared with H. polymorpha.. In addition, optimum methanol concentration as inducer and the effects of carbon sources on AOX1 or MOX promoter repression and secretion efficiency were also studied in both cases.

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References

  1. Dev. Ind. Microbiol. v.29 Development of the methylotrophic yeast, Pichia pastoris, as a host system for the production of foreign proteins Cregg, J.M.;K.R. Madden
  2. Metabolism an gene expression The secretory process and yeast cell-surface assembly, In: The molecular biology of the yeast Saccharomyces Schekman, R.;P. Novick
  3. Ann. Rev. Cell Biol. v.1 Protein localization and membrane traffic in yeast Schekman, R.
  4. Bio/Technology v.5 High-level expression and efficient assembly of hepatitis B surface antigen in the metylotrphic yeast, Pichia pastiros Cregg J.M.;J.F. Tschopp;C. Stillman
  5. Mol. Cell. Biol. v.7 Pichia pastoris as a host system for transformation Cregg, J.M.;K. Hessler
  6. Biotechnology v.11 Recent advances in the expression of foreign genes in Pichia pastoris Cregg, J.M.;C.R. William
  7. Yeast v.5 Size distribution and general structural features of N-linked oligosaccarides from the methylotrophic yeast, Pichia pastoris Grinna, L. S.;J.F. Tschlopp
  8. Biotech. Adv. v.10 Heterologous protein production in yeast Gellissen G.;Z.A. Janowicz;C.P. Hollenberg
  9. Gene expression in methylotrophic yeasts Gellissen G.;C.P. Hollenberg