Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
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Analysis of Taxol and the Related Compound in Exotic Yew (Taxus Spp.)

외래주목의 Taxol 및 관련화합물의 분석

  • Choi, Myung-Suk (Plant Biochemistry Research Unit, Korean Research Institute of Bioscience and Biotechnology) ;
  • Paik, Ki-Hyon (Dept. of Forest Resources, Korea University) ;
  • Kwak, Sang-Soo (Plant Biochemistry Research Unit, Korean Research Institute of Bioscience and Biotechnology)
  • 최명석 (생명공학연구소 식물생화학연구유니트) ;
  • 백기현 (고려대 산림자원학과) ;
  • 곽상수 (생명공학연구소 식물생화학연구유니트)
  • Received : 1999.09.15
  • Published : 1999.09.30
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Abstract

The content of taxol and related compounds in needle and stem tissues of exotic yews growing in the Chollipo Arboretum which located in the West Sea of Korea were determined by HPLC. A considerable variation in contents of taxol and the related compounds within species and among cultivars were observed. The content of taxol was much higher in the needles than those in the young stem tissues except T. X media cv. Vermuleun, T. baccata cv. Washingtonii, and T. X cuspidata cv. Nana, and the content of 10-deacetyl baccatin III(10-DAB III) was also slightly higher in the needle. The needle of T. cuspidata cv. Nigra collected from April was contained the high amount of taxol(1,027 ${\mu}g$ per g dry weight) and also contained high quantity in 10-DAB III(267 ${\mu}g$ per g dry weight). Concentration of taxol in needle was significantly greater than those of meristemic and ovary tissues, Exotic yews growing at the Chollipo Arboretum containing T. cuspidata cv. Nigra could be suitable materials as a source for the tissue and cell cultures for production of taxol.

우리나라에 식재된 외래주목의 taxol 및 전구체인 10-deacetyl baccatin III(10-DAB III)의 함랑을 조사하기 위해 천리포수목원에 식재된 외래주목의 잎과 줄기로부터 추출하여 HPLC로 정량분석하였다. 외래주목의 잎과 줄기의 taxol 함량은 종간 및 종내, 그리고 부위에 따라 상당한 변이를 보였다. Taxol 함량은 T. X media cv. Vcrmulcun, T. baccata cv. Washingtonii, T. X cuspidata cv. Nana을 제외하고 잎에서 놓았으며, 전구체인 10-deacetyl baccatin III(10-DAB III)는 모든 종의 잎에서 높았다. 그 중 4월에 채집한 T. cuspidata cv. Nigra의 잎의 taxol 함량은 건물중 당 1,027 ${\mu}g$, 10-DAB III 의 함량은 건물중 당 267 ${\mu}g$로 보고된 태평양주목의 함량보다 월등히 높았다. 이종의 부위별 tax이 함량은 생장점 및 자방 조직보다 잎에서 높았으며, 이 결과들은 조직 및 세포배양의 재료로 널리 이용할 수 있을 것이다.

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