The Korean Journal of Pain

The Korean Pain Society (대한통증학회)
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L-NAME Inhibits Hyperalgesia Induced by Freund's Complete Adjuvant in Rat Paw

L-NAME에 의한 쥐의 발바닥에서 Freund's Complete Adjuvant에 의해 유발된 통증 억제

  • Lee, Cheong (Department of Anesthesiology, University of Ulsan College of Medicine) ;
  • Choi, Yoon (Department of Anesthesiology, University of Ulsan College of Medicine) ;
  • Song, Myung-Hee (Department of Anesthesiology, University of Ulsan College of Medicine) ;
  • Leem, Joong-Woo (Department of Physiology, University of Yonsei College of Medicine) ;
  • Lee, Dong-Myung (Department of Anesthesiology, University of Ulsan College of Medicine) ;
  • Raja, Srinivasa N. (Department of Anesthesiology/Critical Care Medicine, Johns Hopkins University School of Medicine)
  • 이청 (울산대학교 의과대학 마취과학교실) ;
  • 최윤 (울산대학교 의과대학 마취과학교실) ;
  • 송명희 (울산대학교 의과대학 마취과학교실) ;
  • 임중우 (연세대학교 의과대학 생리학교실) ;
  • 이동명 (울산대학교 의과대학 마취과학교실) ;
  • Published : 1998.10.10
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Abstract

Background: Effect of nitric oxide on the hyperalgesia induced by inflammation is controversial. We attempted to find out the peripheral effects of nitric oxide (NO) on hyperalgesia induced by Freund's complete adjuvant (FCA) induced inflammation. Methods: Male Sprague Dawley rats were divided into three groups; control, low dose NG-nitro-L-arginine methyl ester (L-NAME, 500 ug), high dose L-NAME (5 mg). Inflammation was induced by injecting 0.1 ml of FCA intraplantarly, which shows typical hyperalgesia within twelve hours after injection and maintained for about one week. Drugs were injected 2 hours before, just before, and 3, 6, 9, 12 hours after the injection of FCA. Effect of L-NAME on hyperalgesia was assessed by measuring mechanical hyperalgesia and spontaneous pain for 3 days. Results: When injected at the site of inflammation, L-NAME caused dose dependent reduction of spontaneous hyperalgesia. Mechanical hyperalgesia was also reduced by high dose L-NAME (p<0.05). After systemic injection of high dose L-NAME in the back, no significant difference was noticed. Conclusions: This suggest that L-NAME reduces FCA induced hyperalgesia via peripheral action.

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Acknowledgement

Supported by : 안산 생명과학연구소