Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Plant Regeneration from Seed-Derived Callus in Orchardgrass (Dactylis glomerata L.)

Orchardgrass의 종자유래 캘러스로부터 식물체 재분화

  • Lee, Hyoshin (College of Agriculture, Kyungpook National University) ;
  • Kwon, Yongsham (College of Agriculture, Kyungpook National University) ;
  • Lee, Byung-Hyun (College of Agriculture, Kyungpook National University) ;
  • Lee, Sanghyun (College of Agriculture, Kyungpook National University) ;
  • Jo, Jinki (College of Agriculture, Kyungpook National University)
  • Published : 1998.12.30
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Abstract

aThis experiment was carried out to obtain the basic information on some factors affecting callus formation and plant regeneration in seed culture of orchardgrass. Varietal difference in callus growth and plant regeneration was obvious among four varieties of orchardgrass. "Amba" showed a relatively high capacity for plant regeneration. The $N_6$ medium was superior to MS and $B_5$ in callus formation and plant regeneration. The fresh weight of callus was promoted by the increase of dicamba concentration. However, the maximum frequency (30.0%) of plant regeneration was obtained from the callus formed in presence of 3 mg/L dicamba.

Orchardgrass의 종자배양에서 캘러스형성과 식물체 재분화에 적합한 조건을구명하기 위하여 품종간의 차이와 배지조성 및 생장조절물질의 조성 등에 대한 일련의 실험을 수행한 바, 공시품종중 "Amba"의 식물체 재분화율이 가장 높았고, $N_6$ 배지가 MS, $B_5$ 배지보다 종자에서 형성된 캘러스 생체중이 무거웠으며, 식물체 재분화율도 높게 나타났다. 또한 3 mg/L의 dicamba가 첨가된 배지에서 형성된 캘러스를 재분화배지에 이식하였을 때 식물체 재분화율이 30%로 가장 높게 나타났다.

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