Chromosome 3p Deletions in Korean Head and Neck Carcinomas

한국인 두경부암 환자에서 제3번 염색체 단완의 결손

  • Son Mi-Na (Department of Internal Medicine, Korea University College of Medicine) ;
  • Yoo Young-A (Department of Internal Medicine, Korea University College of Medicine) ;
  • Cho Zeung-Keun (Department of Internal Medicine, Korea University College of Medicine) ;
  • Choi Kun (Department of Otolaryngology, Korea University College of Medicine) ;
  • Choi Jong-Wook (Department of Otolaryngology, Korea University College of Medicine) ;
  • Kim Yeul-Hong (Department of Internal Medicine, Korea University College of Medicine) ;
  • Kim Jun-Suk (Department of Internal Medicine, Korea University College of Medicine)
  • 손미나 (고려대학교 의과대학 내과학교실) ;
  • 유영아 (고려대학교 의과대학 내과학교실) ;
  • 조증근 (고려대학교 의과대학 내과학교실) ;
  • 최건 (고려대학교 의과대학 이비인후과학교실) ;
  • 최종욱 (고려대학교 의과대학 이비인후과학교실) ;
  • 김열홍 (고려대학교 의과대학 내과학교실) ;
  • 김준석 (고려대학교 의과대학 내과학교실)
  • Published : 1998.05.01

Abstract

Objectives: Deletion in the short arm of chromosome 3 is common in many human cancers, including sporadic and hereditary renal carcinomas, small cell lung carcinomas, non-small cell lung carcinomas, and carcinomas of the ovary, breast, and cervix. A high frequency of chromosomal aberrations in head and neck cancers involving chromosome 3p has also been reported. These findings suggest that multiple tumor suppressor genes may be present on the short arm of chromosome 3. Materials and Methods: To investigate the possibility of chromosome 3p deletions in the Korean head and neck cancer patients, we applied a polymerase chain reaction(PCR)-based Restriction Fragment Length Polymorphism analysis to the DNA samples of matched normal mucosa and head and neck squamous cell carcinomas from 19 patients. Results: In the 19 normal samples heterozygosity at the polymorphic loci varied: 6 at the D3F15S2 locus(on telomeric 3p21), 2 at the D3S32 locus(on centromeric 3p21), and 4 at the THRB locus(on centromeric 3p24). In 12 matched carcinoma specimens, LOH(loss of heterozygosity) was observed at D3F15S2 in 1 of 6(17%), D3S32 in 1 of 2(50%), and at THRB in 2 of 4 cases(50%). Conclusion: The frequency of chromosome 3p deletion in the Korean head and neck carcinomas appear as other country did.

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