Restorative Dentistry and Endodontics
- Volume 23 Issue 1
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- Pages.43-53
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- 1998
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- 2234-7658(pISSN)
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- 2234-7666(eISSN)
UREASE ACTIVITY OF STREPTOCOCCUS SALIVARIUS
Streptococcus salivarius의 요소분해효소 활성에 관한 연구
- Chung, Sang-Baek (Department of Conservative Dentistry, Division of Dentistry, Graduate School Kyung-Hee University) ;
- Choi, Ho-Young (Department of Conservative Dentistry, Division of Dentistry, Graduate School Kyung-Hee University) ;
- Min, Byung-Soon (Department of Conservative Dentistry, Division of Dentistry, Graduate School Kyung-Hee University) ;
- Park, Sang-Jin (Department of Conservative Dentistry, Division of Dentistry, Graduate School Kyung-Hee University) ;
- Lee, Jin-Yong (Department of Conservative Dentistry, Division of Dentistry, Graduate School Kyung-Hee University) ;
- Choi, Ki-Woon (Department of Conservative Dentistry, Division of Dentistry, Graduate School Kyung-Hee University)
- 정상백 (경희대학교 치과대학 치과보존학교실) ;
- 최호영 (경희대학교 치과대학 치과보존학교실) ;
- 민병순 (경희대학교 치과대학 치과보존학교실) ;
- 박상진 (경희대학교 치과대학 치과보존학교실) ;
- 이진용 (경희대학교 치과대학 치과보존학교실) ;
- 최기운 (경희대학교 치과대학 치과보존학교실)
- Published : 1998.04.07
Abstract
Dental caries is induced by organic acids produced by oral bacteria. In order to prevent dental caries, therefore, it is essential to maintain neutral pH in the oral cavity. Urea plays a major role in oral pH homeostasis. Urea is hydrolyzed by bacterial ureases to ammonia, causing a pH elevation. Streptococcus salivarius has been shown to be a major contribution to oral ureolysis. Synthesis of urease by S. salivarius appears to be constituitive, but can be greatly enhanced by low pH. It is, therefore, conceivable that ureolytic activity of S. salivarius from a carious lesion is greater than that of the bacterium from a healthy tooth. In the present study, urease activity of S. salivarius isolates from dental plaque of carious lesions was compared with that of the isolates from plaques of the teeth and the dorsum of the tongue; 45 S. salivarius strains were isofated from carious lesions(>C2) of 21 individuals with dental caries and 30 strains from 10 individuals without dental caries. The results were as follows: 1. All the 21 individuals with dental caries harbored ureolytic S. salivarius whereas 3 of 13 individuals without dental caries harbored non-ureolytic strains of S. salivarius. 2. All the 45 S. saliuarius isolates from carious lesions showed urease activity. In contrast, of 30 isolates from individuals without dental caries, 17 isolates(56.7%) did not demonstrate urease activity, or if any, very little(<5
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