Korean Journal of Microbiology (미생물학회지)
- Volume 34 Issue 3
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- Pages.82-86
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- 1998
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
An alkaline proteinase produced by Yarrowia lipolytica 504D
Yarrowia lipolytica 504D의 Alkaline Proteinase 특성
- Kim, Chang-Hwa (Department of Microbiology, Kyungpook National University) ;
- Jin, Ingnyol (Department of Microbiology, Kyungpook National University) ;
- Yu, Choon-Bal (Department of Food Science and Technology, Taegu University)
- Received : 1998.05.20
- Accepted : 1998.08.21
- Published : 1998.09.01
Abstract
An alkaline proteinase secreted from Yarrowia lipolytica 504D was purified by salting-out and column chromatography. The molecular weight of the purified enzyme was about 32,000 Da estimated by SDS-PAGE. The optimal condition for the activity of the enzyme was at pH 9.5 and
Yarrowia lipolytica 504D가 생산하는 alkaline proteinase를 정제한 결과, 분자량은 32,000으로 나타났고, pH 9.5와