Korean Journal of Veterinary Research (대한수의학회지)
- Volume 38 Issue 1
- /
- Pages.139-145
- /
- 1998
- /
- 2466-1384(pISSN)
- /
- 2466-1392(eISSN)
In vitro isolation of a bovine Neospora in Korea
국내 소에서 Neospora caninum의 분리
- Kim, Jae-hoon (National Veterinary Research Institute) ;
- Sohn, Hyun-joo (National Veterinary Research Institute) ;
- Hwang, Eui-kyung (National Veterinary Research Institute) ;
- Hwang, Woo-suk (College of Veterinary Medicine, Seoul National University) ;
- Hur, Kwon (College of Veterinary Medicine, Seoul National University) ;
-
Jean, Young-hwa
(National Veterinary Research Institute)
;
-
Lee, Byung-chun
(College of Veterinary Medicine, Seoul National University)
;
- Rhee, Jae-chin (National Veterinary Research Institute) ;
- Kang, Yung-bai (National Veterinary Research Institute) ;
- Yamane, Itsuro (Laboratory of Epidemiology, National Institute of Health) ;
-
Kim, Dae-yong
(College of Veterinary Medicine, Seoul National University)
- 김재훈 (수의과학연구소) ;
- 손현주 (수의과학연구소) ;
- 황의경 (수의과학연구소) ;
- 황우석 (서울대학교 수의과대학) ;
- 허권 (서울대학교 수의과대학) ;
-
진영화
(수의과학연구소)
;
-
이병천
(서울대학교 수의과대학)
;
- 이재진 (수의과학연구소) ;
- 강영배 (수의과학연구소) ;
- 야마네 이츠로 (일본 가축위생시험장) ;
-
김대용
(서울대학교 수의과대학)
- Received : 1998.01.13
- Published : 1998.03.25
Abstract
The Neospora sp. was isolated from the brain of 1 calf via continuous in vitro cultivation in Vero cell. Neospora tachyzoites were observed 45 days after inoculation of the homogenized brain suspension into the Vero cell. The isolated parasite (named tentatively as NCKB-1) was morphologically and ultrastructurally similar to the previously reported Neospora sp isolated in cattle (BPA-1, JPA-1). A comparison of the antigenic reactivity of cultivated tachyzoites with polyclonal antisera to Neospora caninum and Toxoplasma gondii confirmed that this protozoal isolate was similar to N caninum. This is the first report of successful isolation of Neospora sp from cattle in Korea.