Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Gene typing of staphylococcal superantigens produced by Staphylococcus aureus isolates from meat

식육유래의 Staphylococcus aureus으로부터 산생되는 staphylococcal superantigens의 유전자형 분석

  • Yoon, Jang-won (Department of Microbiology, College of Veterinary Medicine, Seoul National University) ;
  • Jung, Suk-chan (National Veterinary Research and Quarantine Services) ;
  • Yang, Soo-jin (Department of Microbiology, College of Veterinary Medicine, Seoul National University) ;
  • Jung, Byong-youel (National Veterinary Research and Quarantine Services) ;
  • Seo, Keon-suk (Department of Microbiology, College of Veterinary Medicine, Seoul National University) ;
  • Kim, So-hyun (Department of Microbiology, College of Veterinary Medicine, Seoul National University) ;
  • Park, Yong-ho (Department of Microbiology, College of Veterinary Medicine, Seoul National University)
  • 윤장원 (서울대학교 수의과대학 미생물학교실) ;
  • 정석찬 (농림부 국립수의과학검역원) ;
  • 양수진 (서울대학교 수의과대학 미생물학교실) ;
  • 정병열 (농림부 국립수의과학검역원) ;
  • 서근석 (서울대학교 수의과대학 미생물학교실) ;
  • 김소현 (서울대학교 수의과대학 미생물학교실) ;
  • 박용호 (서울대학교 수의과대학 미생물학교실)
  • Received : 1998.09.11
  • Published : 1998.09.25
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Abstract

Using the previous established multiplex PCR (mPCR), the presence of six kinds of staphylococcal superantigen (SAg) genes was investigated for nineteen Staphylococcus aureus isolates from the commercialized meat sources. As a result, only one isolate from pork among 19 S aureus isolates (5.3%) was confirmed as a potential SAg producer and harbored sec gene. The results in this study suggest that meat may not be major contagion of staphylococcal food poisoning (SFP) in Korea and that staphylococcal enterotoxin type C may be associated with the disease. Also, the mPCR method in this study can be a useful genotypic method which can overcome the typical disadvantages of conventional antibody-based methods due to antigenic homology, and furthur survey on food-borne S aureus isolates can provide the important epidemiological data for SFP in Korea.

Keywords

Acknowledgement

Supported by : 서울대학교 수의과대학 부설 수의과학연구소