대한수의학회지 (Korean Journal of Veterinary Research)

  • 제38권4호
  • /
  • Pages.771-776
  • /
  • 1998
  • /
  • 2466-1384(pISSN)
  • /
  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
권호 표지

Streptococcus suis 신속동정을 위한 PCR 기법

Polymerase chain reaction for a rapid and specific identification of Streptococcus suis

  • 정병열 (농림부 수의과학검역원 세균과) ;
  • 정석찬 (농림부 수의과학검역원 세균과) ;
  • 김종염 (농림부 수의과학검역원 세균과) ;
  • 박용호 (서울대학교 수의과대학) ;
  • 김봉환 (경북대학교 수의과대학)
  • Jung, Byeong-yeal (Department of Bacteriology, National Veterinary Research and Quarantine Service, MAF) ;
  • Jung, Suk-chan (Department of Bacteriology, National Veterinary Research and Quarantine Service, MAF) ;
  • Kim, Jong-yeom (Department of Bacteriology, National Veterinary Research and Quarantine Service, MAF) ;
  • Park, Yong-ho (College of Veterinary Medicine, Seoul National University) ;
  • Kim, Bong-hwan (College of Veterinary Medicine, Kyungpook National University)
  • 투고 : 1998.05.19
  • 발행 : 1998.12.22
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초록

Synthetic oligonucleotide primers of 20 and 21 bases, respectively, were used in the polymerase chain reaction (PCR) to amplify a sequence of the mrp gene, which encodes the muramidase released protein of Streptococcus suis. Amplification was not recorded when 5 other streptococcal species were tested or when 9 different nonstreptococcal species were tested. A DNA fragment of 517bp was amplified from the genomic DNA of S suis. The lower detection limit was 100pg of the genomic DNA. The primers recognized 34 serotypes of S suis reference strains and 9 isolates from pneumonic lung, brain, nasal discharge, tonsil. This results suggest that the amplification of the mrp gene by PCR method is potential for the identification of S suis isolates.

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