Histological and immunohistochemical studies on the duodenum of the chicken embryos

닭 태자의 십이지장에 대한 조직학적 및 면역조직화학적 연구

With histological changes, ontogeny and relative frequencies of bovine Sp-1/chromogranin(bCG)-, serotonin-, gastrin-, cholecystokinin-8(CCK-8)-, somatostatin-, S-100 protein-, polypeptide YY(PYY)- and glucagon-immunoreactive cells were investigated in the duodenum of the chicken embryos from 10 days of incubation to hatching. Histologically, pseudostraitified columnar epithelium were observed from 10 days of incubation to 14 days of incubation, thereafter these epithelium were differentiated to simple columnar epithelium. $Liberk{\ddot{u}}hn$ glands were observed from 18 days of incubation and goblet cells were detected from hatching. In the duodenum, bCG-immunoreactive cells were detected from 14 days of incubation and increased to 18 days of incubation, thereafter decreased with ages. Serotonin-immunorecative cells were detected from 14 days of incubation and increased with ages. Somatostatin-immunoreactive cells were detected from 14 days of incubation and CCK-immunoreactive cells were detected from 19 days of incubation. No gastrin-, S-100 protein-, PYY-, glucagon-immunoreactive cells were detected in this study.