Asian-Australasian Journal of Animal Sciences
- Volume 11 Issue 5
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- Pages.498-502
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- 1998
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- 1011-2367(pISSN)
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- 1976-5517(eISSN)
DOI QR Code
Quick Detection of Firefly Luciferase Gene Expression in Live Developing Bovine Embryos by Photoncounting
- Nakamura, A. (Department of Biological Resources and Environmental Sciences, School of Agricultural Sciences, Nagoya University) ;
- Okumura, J. (Department of Biological Resources and Environmental Sciences, School of Agricultural Sciences, Nagoya University) ;
- Muramatsu, T. (Department of Biological Resources and Environmental Sciences, School of Agricultural Sciences, Nagoya University)
- Received : 1997.08.25
- Accepted : 1998.03.10
- Published : 1998.10.01
Abstract
The present study was designed, fIrst to develop the new methodology to measure the bioluminescence activity easily in live developing bovine embryos by photoncounting, and secondly to compare the expression efficiency of four luciferase reporter genes in bovine embryos at four- to 16-cell stages. In experiment 1, equimolar pSVlacZ and pSVEluc were microinjected into the pronucleus of fertilized bovine oocytes. At 2 days after micro injection, bioluminescence activity of these embryos was measured by photoncounting with a luminometer for 1 min, and lacZ gene expression in the same embryos was assayed by X-gal staining. All the luciferase-positive oocytes showed some bacterial