Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
권호 표지

Study on analysis method of herbicide quizalofop-ethyl

제초제 quizalofop-ethyl 분석법에 관한 연구

  • Kim, Hee-Kwon (Chonnam Provincial Rural Development Administration Plant Environ mental Division) ;
  • Kim, Byeong-Ho (Chonnam Provincial Rural Development Administration Plant Environ mental Division) ;
  • Shim, Jae-Han (Department of Agricultural Chemistry,Chonnam National University) ;
  • Shu, Yong-Tack (Department of Agricultural Chemistry,Chonnam National University)
  • 김희권 (전남농촌진흥원식물환경과) ;
  • 김병호 (전남농촌진흥원식물환경과) ;
  • 심재한 (전남대학교 농과대학 농화학과) ;
  • 서용택 (전남대학교 농과대학 농화학과)
  • Published : 1998.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

These studies were conducted to develope analysis method of herbicide quizalofop-ethyl by Gas Liquid Chromatography(GLC) and Enzyme-Linked Immunosoment Assay(ELISA) in soil and plant. Quizalofop produced by hydrolysis of quizalofop-ethyl was conjugated with bovine serum albumin(BSA). Quizalofop antibody was developed in rabbits by using BSA conjugation. Antibody titer, incubation temperature, and incubation time was 32,000, $37^{\circ}C$ and 4hours respectively. Minimum detection limit of quizalofop-ethyl by ELISA was 5ppb. Quizalofop-ethyl recovery from soil by ELISA was more than 95percent. Minimum detection limit of quizalofop-ethyl by GLC was 5ppb. Quizalofop-ethyl recovery from soil by GLC was from 89 percent to 100 percent. Minimun detection limit of quizalofop-ethyl by HPLC was 100ppb. Quizalofop-ethyl recovery from soil by HPLC was 89.6 percent.

종래에 사용해 오던 HPLC에 의한 quizalofop-ethyl의 분석법을 개선시키고, 또한 ELISA와 GLC를 이용한 새로운 quizalofop-ethyl분석법을 개발하고자 시험을 수행한 결과는 다음과 같다. ELISA 실험에서 적정배양온도는 $37^{\circ}C$였으며 배양시간은 4시간이었다. Coating antigen의 적정수준은 $20{\mu}l/ml$이며 검출 한계는 5ppb이고 회수율은 95%이상이었다. 또 GLC와 HPLC에 의한 검출한계는 5ppb, 100ppb였으며, 토양에서의 회수율은 95.4, 89.5% 이상으로 나타났다. 이상에서 보는 바와 같이 ELISA와 GLC에 의한 quizalofop-ethyl분석법이 HPLC에 의한 것보다 양호한 것으로 나타났다.

Keywords