Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Irradiation in Central Nervous System and Recovery Effect: Action of Antioxidants and NMDA-receptor Antagonists

중추신경의 방사선 조사와 회복효과: 항산화제와 NMDA-receptor 길항제의 작용

  • Mun, Yeun-Ja (Department of Anatomy, School of Medicine, Wonkwang University) ;
  • Park, Seung-Taeck (Department of Anatomy, School of Medicine, Wonkwang University) ;
  • Choi, Min-Kyu (Department of Anatomy, School of Medicine, Wonkwang University) ;
  • Jeong, Dong-Hyeok (Department of Radiation Oncology, School of Medicine, Wonkwang University) ;
  • Moon, Sun-Rock (Department of Radiation Oncology, School of Medicine, Wonkwang University) ;
  • Chung, Yeun-Tai (Department of Anatomy, School of Medicine, Wonkwang University)
  • 문연자 (원광대학교 의과대학 해부학교실) ;
  • 박승택 (원광대학교 의과대학 해부학교실) ;
  • 최민규 (원광대학교 의과대학 해부학교실) ;
  • 정동혁 (원광대학교 의과대학 방사선종양학 교실) ;
  • 문성록 (원광대학교 의과대학 방사선종양학 교실) ;
  • 정연태 (원광대학교 의과대학 해부학교실)
  • Published : 1998.12.01
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Abstract

The neurotoxic effects of radiation have been studied in NSC-34 hybrid cells derived from embryonic mouse spinal cord cells. NSC-34 cells irradiated at 25Gy were decreased the cell viability in a time and dose dependent manner. The decrease in cell viability induced by the irradiation was blocked by catalase. Antagonists of the N-methyl-D-aspartate (NMDA) receptor, including D-2-amino-5-phosphonovaleric acid (APV) and chlorokynurenic acid (CKA), similarly blocked radiational induced in cell viability. We performed morphological analysis of light and electron microscope. NSC-34 cells irradiated at 25Gy were decreased the cell density and increased lysosomes and vacuoles in the cytoplasm. Especially chromatin modification was observed. These results indicated that radiation was involved in the oxidant-initiated neurotoxicity and the compounds catalase, APV and CKA were shown to be neuroprotective against radiation.

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