Journal of Korean Society of Forest Science (한국산림과학회지)
- Volume 87 Issue 2
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- Pages.260-269
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- 1998
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- 2586-6613(pISSN)
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- 2586-6621(eISSN)
In vitro Cytotoxin Activity of Urushiol in the Sap of Rhus verniciflua STOKES
옻나무 칠액성분(漆液成分) 중 Urushiol의 암세포(癌細胞) 증식억제(增殖抑制) 효과(效果) - in vitro 세포독성효과(細胞毒性效果) -
- Na, Chun-Soo (Forestry Genetics Research Institute) ;
- Jung, Nam-Chul (Forestry Genetics Research Institute) ;
- Oh, Kwang-In (Dept. of Forestry, Chonnam National University)
- Received : 1998.03.03
- Published : 1998.06.30
Abstract
This study was conducted to screen the biological activity of urushiol in the sap of lac tree(Rhus verniciflua STOKES) which has been used in traditional folk remedies. Cytotoxic activity of urushiol was screened with L1210(mouse luekemia cell), PC-9(human lung adenocarcinoma cell), A427(human lung adenocarcinoma cell) and KATO III (human stomach adenocarcinoma cell) The stepwise hexane : acetone eluent fractions of the urushiol were obtained by the silica gel adsorption column chromatography and added to the culture media containing L1210, PC-9. A427, and KATO III, respectively. A hexane : acetone(90 : 10, v/v) eluent fraction of them showed the lowest 50% inhibition concentration(
본 연구는 전통적인 민간요법으로 많이 이용되고 있는 옻나무의 옻칠액의 주성분인 urushiol의 암세포증식억제 효과를 검정하기 위하여 L1210(mouse 혈액암세포), PC-9(인체폐암세포), A427(인체폐암세포), KATO III(인체위암세포)에 대한 urushiol의 세포독성을 검정하였다. Urushiol은 column chromatography에서 hexane과 acetone을 혼합하여 step-wise법으로 용출한 분획을 암세포주 L1210, PC-9, A427, KATO III에 투여했다. 그 결과 암세포 증식억제효과는 hexane : acetone(9 : 1) 분획이 가장 높았고, 그외 50%억제농도(
Keywords
- Urushiol;
- Tetraplatin;
- $IC_{50}({\mu}g/m{\ell})$;
- Cancer cell lines(L1210, PC-9, A427, KATO III, A427)