YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Flow Cytometrical Analysis of the Antitumor and Immunomodulatory Activities of GLB-A and GLB-B, the Protein-polysaccharide Fractions of the Growing Tips of Ganoderma Lucidum

영지버섯 생장점 단백다당체 GLB-A, GLB-B의 항암효과 및 면역 활성에 관한 유세포 분석학적 연구

  • Oh, Jung-Yeon (Laboratory of Microbial Chemistry, College of Pharmacy, Chung-Nam National University) ;
  • Chung, Kyeong-Soo (Laboratory of Microbial Chemistry, College of Pharmacy, Chung-Nam National University)
  • 오정연 (충남대학교 약학대학 미생물약품화학교실) ;
  • 정경수 (충남대학교 약학대학 미생물약품화학교실)
  • Published : 1998.10.01
Download PDF
⟨ Previous Next ⟩

Abstract

In the previous study we described the antitumor effect of GLB, a protein-polysaccharide fraction separated from the growing tips of Ganoderma lucidum, against sarcoma 18 0 solid tumor in ICR mice. In this study, we separated an acidic protein-polysaccharide fraction, GLB-A, and a basic protein-polyaccharide fraction, GLB-B, from GLB by differential precipitation, and elucidated their antitumor and immunomodulatory activities. When ip injected at the dose of 50mg/kg/day into the ICR mice, GLB-A and GLB-B inhibited the growth of ip implantated sarcoma 180 cells by 32.4% and 21.0%, respectively. Of these, GLB-A increased the % lymphoblast in the spleen of the tumor-bearing and the normal mice by 20.9% and 123.0%, and the CD4/CD8 ratio by 73.3% and 22.4%, respectively. GLB-A also increased the expression of CD25 (IL-2 receptor alpha ch0ain) in normal mice by 82.0%. These results strongly suggest that GLB-A is a promising candidate for antitumor immunomodulatory medicine.

Keywords

References

  1. Clin. Exp. Immunol. v.104 Influence of the mouse Bcq. Tbc-1 and xid genes on resistance and immune responses to tuberculosis infection and immune responses to tuberculosis infection and efficacy of Bacille Calmette-Guerin (BCG) vaccination. Nikonenko, B. V.;Apt, A. S.;Mezhlumova, M. B.;Avdienko, V. G.;Yereneev, V. V.;Moroz, A. M.
  2. Nippon Nokeikagaku Kaishi v.59 Fraction, chemical modification and antitumor activity of water-soluble polysaccharide of the fruiting body of Ganoderma lucidum. Mizuno, T.;Suzuki, E.;Maki, K.;Tamaki, H.
  3. Cancer Res. v.30 Fraction of the polysaccharide with antitumor activity, especially lentinan from the Lentinus edodes (an edible mushroom). Chihara, G.;Hamura, J.;Maeda, Y. Y.;Arai, Y.;Fukuoka, F.
  4. Gann. v.65 Protein bound polysacchride preparation, PS-K, effective against mouse sarcoma 180 and rat ascites hepatoma AH-13 by oral use. Tsukagoshi, S.;Ohasi, F.
  5. v.60 Host-mediated antitumor action of schizophyllan. a glucan produced by Schizophyllum commune. Gann. Komatsu, N.;Okubo, S.;Likumoto, S.;Kimura, K.;Saito, G.;Sasaki, S.
  6. v.35 Potentiation of host-mediated antitumor activity in mice by β-glucan obtained from Griofola frondosa. Chem. Pharmaceu. Bull. Adachi, K.
  7. v.12 Antitumor activity of Sarcodon asparatus (Berk.) S. Ito. and ganoderma lucidum (Fr.) Karst. J. Phamacobiodyn. Maruyama, H.;Yamazaki, K.;Murofushi, S.;Konda, C.;Ikekawa, T.
  8. Biochem. Biotech. Biochem. v.57 Antitumor active polysaccharides from the chinese mushroom shongshan Ling Zhi, the fruiting body of Ganoderma tsugae Wang, G.;Zhang, J.;Mizuno, T.;Zhuang, C.;Hitoshi, I.;Mayuzumi, H.;Okamoto, H.;Li, J.
  9. Study of the anti inflammatory effect of Ganoderma lucidum. Recent advances in Ganoderma lucidum research. The Phamaceutical Society of Korea Stavinoha, W. B.;Satsangi, N.;Weintraub, S. T.
  10. Chung-Kuo-Chung-Hsi-I-Chieh-Ho-Tsa-Chih v.13 Effect of Japanese Ganoderma lucidum on production of IL-2 from murine splenocytes. Zhang, L. X.;Mong, H.;Zhou, X. B.
  11. Anticancer Res. v.12 The effect of Ganoderma lucidum on induction of differentiation in leukemic U937 cells. Lieu, C. W.;Lee, S. S.;Wang, S. Y.
  12. Transplantation v.60 Ling Zhi-8(LZ-8): Studies of a new immunonodulating agent. Kino, K.;Hem, L. G.;Vliet, J. A.;Bocken, C. F.;Hoitsma, A. J.;Tax, W. J.
  13. v.41 Immunoactivities of the protein-polysaccharides of the tips of the carpophores of Ganoderma lucidum. Yakhak Hoeji. Chung, K. S.;Kim, S. B.;Chung, S. H.
  14. Methods in Microbiology(Vol. 5B). Norris, J. R.;Robbons, D. W.(Eds.)
  15. Anal. Biochem. v.79 A rapid, sensitive and versatile assay for protein using Coomassie brilliant blue G250. Sedmak, J. J.;Grossberg, S. E.
  16. Current Protocols in Immunology: Survival surgery, removal of the spleen and thymus. Reeves, J. P.;Reeves P. A.
  17. Immunology. Kuby, J.