YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

The Mechanism of Resistance to Rifampicin in Bifidobacterium bifidum

Bifidobacterium bifidum에서 리팜피신에 대한 내성기전

  • 정영자 (식품의약품안전청 생약규격과) ;
  • 박성수 (식품의약품안전청 생약규격과) ;
  • 백문창 (서울대학교 약학대학) ;
  • 김병각 (서울대학교 약학대학) ;
  • 최응칠 (서울대학교 약학대학)
  • Published : 1998.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

Bifidobacterium bifidum OFR9 that exhibits acquired resistance to rifampicin and fluoroquinolones was selected by MNNG and multi-step mutation method. To investigate the resistance mechanism to rifampicin in the strain, RNA polymerase from B. bifidum parent strain and rifampicin-resistance OFR9 was partially purified and its sensitivity to rifampicin was assayed. The profile of RNA polymerase preparation of B. bifidum parent and B. bifidum OFR9 is similar to that of E. coli RNA polymerase that includes the basic subunits of ${\beta}$`, ${\beta},\;{\sigma},\;{\alpha}$ but which are a little different in size when they are compared with E. coli RNA polymerase subunits. RNA polymerase isolated from the parent strain was inhibited by 1${\mu}$g/ml rifampicin but that from B. bifidum OFR9 was not affected by 100${\mu}$g/ml concentration of rifampicin. RNA polymerase activity of B. bifidum OFR9 was maintained over 90% through that rifampicin concentration. This result is consistent with MIC values of in vitro test. It can be concluded that the mechanism of rifampicin resistance in B. bifidum OFR9 is due to an alteration of RNA polymerase.

Keywords

References

  1. J. Infect. Dis. v.167 A ribosomal gene mutation in streptomycin resistant Mycobacterium tuberculosis isolates Douglass, J.;Steyn, L.M.
  2. Amtimicrob. Agents Chemother v.37 Molecular basis of rifampin resistance in Mycobacterium leprae Honore, N.;Cole, S.T.
  3. J. Infect. Dis. v.169 The neglected global tuberculosis problem: A report of the 1992 world congress on tuberculosis Sinder, D.E.;Montagne, J.R.
  4. J. Gen. Appl. Microbiol. v.43 The effects of Bifidobacterium bifidum OFR9, a strain resistant to antituberculosis and antileprosy agents, on fecal flora in mice Chung, Y.J.;Kim, S.K.;Choi, E.C.
  5. Biochem. v.18 Purification and properties of the α subunit of Escherichia coli DNA-dependent RNA polymerase Lowe, P.A.;Hager, D.A.;Burgess, R.R.
  6. Nature v.277 Cleavage of structural proteins furing the assembly of the head of bacteriophage T4 Laemmli, U.K.
  7. J. Bacteriol. v.122 Rifampin resistance mutation of Bacillus subtilis altering the electrophoretic mobility of the β-subunit of ribonucleic acid polymerase Linn, T.;Losick, R.;Sonenshein, A.L.
  8. Cell v.16 Utilization of promoter and terminator sites on bacteriophage T7 DNA by RNA polymerases from a variety of bacterial orders Wiggs, J.L.;Brush, J.W.;Chamberlin, M.J.
  9. Mol. Microbiol. v.8 Mycobacterium smegmatis RNA polymerase: DNA supercoiling action to rifampicin and mechanism of rifampicin resistance Levin, M.E,;Hatfull, G.F.
  10. Rev. Infect. Dis v.5 Rifampin: mechanism of action and resistance Wehrli, W.
  11. Mol. Gen. Genet. v.196 Mutation to rafampicin resistance at the begining of the RNA polymerase β subunit gene in Escherichia coli Lisitsyn, N.A.;Sverdlov. E.D.;Moiseyeva, E.P.;Nikiforov, V.G.
  12. Antimicrob. Agents Chemother. v.38 The rpoB gene of Mycobacterium tumerculosis Miller, L.P.;Crawford, J.T.;Shinnick, T.M.
  13. Annu. Rev. Microbiol. v.25 RNA polymerase Burgess, R.R.
  14. J. Bacteriol. v.125 Altered nutritional requirements associated with mutations affecting the structure of ribonucleic acid polymerase in Lactobacillus casei Morishita, T.;Yura, T.
  15. J. Bacteriol. v.108 Mechanism of action of rifampin on Mycobacterium smegmatis White, R.J.;Lancini, G.C.;Silvestri, L.G.
  16. The molecular basis of antibiotic action(2nd ed.) Gale, E.F.;Cundliffe, E.;Richmond, M.H.;Waring, M.J.
  17. Amtimicrob. Agents Chemother v.11 Permeability barrier to rifampin in Mycobacteria Hui, J.;Gordon, N.;Kajiok, R.
  18. J. Biochem. v.67 Purification of DNA-dependent RNA polymerase from Escherichia coli Tada, M.;Tada, M.
  19. Eur. J. Biochem. v.48 Transcription in lactobacillaceae. DNA-dependent RNA polymerase from Lactobacillus curvatus Stetter, K.O.;Zillig, W.