YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Effects of Rhei Rhizoma, Ephedrae Herba and Scutellariae Radix on the Metabolism of 7-Ethoxycoumarin in Isolated Rat Liver

적출관류 간에서 대황, 마황 및 황금이 7-에톡시쿠마린의 대사에 미치는 영향

  • Choi, Ki-Hwan (Korea Food and Drug Administration, National Institute of Toxicological Research) ;
  • Kim, Soon-Sun (Korea Food and Drug Administration, National Institute of Toxicological Research) ;
  • Park, Youn-Joo (Korea Food and Drug Administration, National Institute of Toxicological Research) ;
  • Chung, Hye-Joo (Korea Food and Drug Administration, National Institute of Toxicological Research) ;
  • Ahn, Mee-Ryung (Korea Food and Drug Administration, National Institute of Toxicological Research) ;
  • Seo, Soo-Kyung (Korea Food and Drug Administration, National Institute of Toxicological Research) ;
  • Sheen, Yhun-Yhong (College of Pharmacy, Ewha womans University) ;
  • Kim, Dong-Sup (Korea Food and Drug Administration, National Institute of Toxicological Research) ;
  • Chang, Young-Sup (Korea Food and Drug Administration, National Institute of Toxicological Research)
  • 최기환 (식품의약품안전청 국립독성연구소) ;
  • 김순선 (식품의약품안전청 국립독성연구소) ;
  • 박윤주 (식품의약품안전청 국립독성연구소) ;
  • 정혜주 (식품의약품안전청 국립독성연구소) ;
  • 안미령 (식품의약품안전청 국립독성연구소) ;
  • 서수경 (식품의약품안전청 국립독성연구소) ;
  • 신윤용 (이화여자대학교 약학대학) ;
  • 김동섭 (식품의약품안전청 국립독성연구소) ;
  • 장영섭 (식품의약품안전청 국립독성연구소)
  • Published : 1998.08.01
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Abstract

In order to study the effects of Rhei rhizoma, Ephedrae herba and Scutellariae radix on hepatic metabolism, we examined the pretreatment effect of those on the metabolism of 7-e thoxycoumarin (EC). Water extracts (1g/kg) of Rhei rhizoma, Ephedrae herba and Scutellariae radix were administered orally to rats for 7 days, respectively. Livers were then isolated and perfused with 100mcM EC for 2 hours. The metabolites of EC, 7-hydroxycoumarin, sulfate conjugate and glucuronide conjugate were measured in the perfusates. The amount of glucuronide conjugates was decreased in Rhei rhizoma pretreated rats (p<0.01), however, 7-hydroxycoumarin was increased in Ephedrae herba pretreated rats (p<0.01). We examined whether the change of enzyme activity is related to the change of cytochrome P4501A1 and P4502B1 mRNA level in the perfused rat liver, which are responsible for EC metabolism. CYP1A1 and CYP2B1 mRNA level was increased, which was was not statistically significant with rhei rhizoma nor ephedrae herba pretreatment. We also assessed the hepatotoxicity of Rhei rhizoma, Ephedrae herba and Scutellariae radix. The activities of ALT and AST were assayed at 24 hours after 7 days administration. Only the ratio of ALT over AST was increased in ephedrae herba pretreated rats (p<0.05). Lipid peroxidation was increased in Rhei rhizoma treatment (p<0.05), while histopathological examination performed after liver perfusion did not show any difference compared with vehicle treatment. These results suggest that Ephedrae herba pretreatment increases the o-deethy-lation of 7-ethoxycoumarin in rats, which may be mediated by CYP1A1 mRNA induction.

Keywords

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