YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
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Prediabetic In vitro Model in Pancreatic Beta Cells Induced by Interleukin-$1{\beta}$

췌장 베타세포에서 인터루킨-$1{\beta}$로 유도한 인슐린 의존형 당뇨병 실험 모델

  • Lee, Ihn-Soon (Department of Life Science, University of Science and Technology) ;
  • Lee, In-Ja (College of Pharmacy, Catholic University of Taegu-Hyoseung) ;
  • Kim, Kyong-Tai (Department of Life Science, University of Science and Technology)
  • 이인순 (포항공과대학교 생명과학과) ;
  • 이인자 (효성가톨릭대학교 약학대학) ;
  • 김경태 (포항공과대학교 생명과학과)
  • Published : 1998.08.01
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Abstract

To establish prediabetes in vitro/ model concerning the etiology of Insulin Dependent Diabetes Mellitus (IDDM) in cellular level we have designed experimental prediabefic model in pancreatic beta cells. RINm5F, HIT-T15 and isolated rat islets were chosen as pancreatic beta cells. Since interleukin-$1{\beta}$-induced beta cell cytotoxicity has been implicated in the autoimmune cytotoxicity of IDDM, we used inteleukin-$1{\beta}$ as diabetogenic agent. For establishment of prediabetic in vitro model, the degree of beta cell deterioration was determined by cell proliferation, insulin release and morphological appearance. Cell proliferation, insulin release and morphology were changed dose-dependently in condition that inteleuldn-$1{\beta}$ was exposured to pancreatic beta cells. The concentration and exposure time of interleukin-$1{\beta}$ to set up prediabetic model in beta cell lines and isolated rat islets were 100${\sim}$1000U/ml, 48hr. And 25${\sim}$100U/ml, 48hr, respectively.

Keywords

References

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