Archives of Pharmacal Research
- Volume 21 Issue 6
- /
- Pages.651-656
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- 1998
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- 0253-6269(pISSN)
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- 1976-3786(eISSN)
Determination of Aloesin in Plasma by High-Performance Liquid Chromatography as Fluorescent 9-Anthroyl Derivative
-
Kim, Kyeong-Ho
(College of Pharmacy, Kangwon National University)
;
- Lee, Jin-Gyun (College of Pharmacy, Kangwon National University) ;
-
Park, Jeong-Hill
(College of Pharmacy, Seoul National University)
;
- Shin, Young-Geun (College of Pharmacy, Seoul National University) ;
- Lee, Seung-Ki (College of Pharmacy, Seoul National University) ;
- Cho, Tae-Hyung (Namyang Aloe Co.)
- Published : 1998.12.01
Abstract
A sensitive high-performance liquid chromatographic (HPLC) method for the determination of aloesin in plasma was developed. After solid-phase extraction from plasma and derivatization of aloesin and compound AD-1, which was prepared from aloesin as a internal standard, with 9-anthroylnitrile in the presence of quinuclidine, the derivatives were separated on a Inertsil ODS-3 column using acetonitrile/methanol/water (3:1:6) as a mobile phase, and detected fluorimetrically at 460nm with excitation at 360nm. The detection limit of aloesin was 3.2ng/ml in plasma (S/N=3).