Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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A Study for Acute and Four-week Intravenous Toxicity of Alginase in Rats

랫드에서 Alginase의 급성 및 4주간 정맥 내 반복투여 독성시험에 관한 연구

  • Ihm, Jong-Hee (Department of Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Nam, Jeong-Seok (Department of Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Che, Jeong-Hwan (Department of Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Li, Guang-Xun (Department of Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Lee, Hak-Mo (Department of Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Lee, Won-Woo (Department of Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Yi, Beoung-Hi (Department of Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Jung, Ji-Youn (Department of Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Park, Jae-Hak (Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University) ;
  • Lee, Yong-Soon (Department of Public Health, College of Veterinary Medicine, Seoul National University) ;
  • ,
  • 임종희 (서울대학교 수의과대학 공중보건학실) ;
  • 남정석 (서울대학교 수의과대학 공중보건학실) ;
  • 제정환 (서울대학교 수의과대학 공중보건학실) ;
  • 이광훈 (서울대학교 수의과대학 공중보건학실) ;
  • 이학모 (서울대학교 수의과대학 공중보건학실) ;
  • 이원우 (서울대학교 수의과대학 공중보건학실) ;
  • 이병희 (서울대학교 수의과대학 공중보건학실) ;
  • 정지윤 (서울대학교 수의과대학 공중보건학실) ;
  • 박재학 (서울대학교 수의과대학 실험동물의학교실) ;
  • 이영순 (서울대학교 수의과대학 공중보건학실) ;
  • Published : 1998.09.01
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Abstract

Alginase$Alginase^{ⓡ}$ (Arginine esterase) is one of the snake venoms which is mainly consisted of arginine esterase and acts as a thrombus -forming inhibitor/thrombus-lysin. These present studies were performed to investigate of the acute and subacute toxicity of the Alginase$Alginase^{ⓡ}$ in rats. In acute toxicity study, rats were single administered intravenously with dosages of 0.001, 0.01. 0.1, 1 and 10U/kg B.W. and examined the number of death, clinical sign, body weight and pathological change for 7days after administration of Alginase$Alginase^{ⓡ}$. At maximum dose level (10U/kg B.W.), Alginase$Alginase^{ⓡ}$ induced symptoms of shock with cyanosis and dyspnea. But these symptoms dissappeared after 30~50 minutes and we could not find any other toxic effect in rats. Therefore, $LD_{50}$ Value of Alginase was over 10U/kg B.W. in rats. In four-week intravenous toxicity study of Alginase$Alginase^{ⓡ}$, rats were administered intravenously seven days per week for 28 days, with dosages of 0, 0.0125, 0.125 and 1.25U/kg B.W./day, respectively. Alginase$Alginase^{ⓡ}$ did not caused any death and showed any clinical signs in rats. No significant Alginase$Alginase^{ⓡ}$ -related changes were found in feed uptake, water consumption, hematology, serum biochemistry, urinalysis, ocular examination, organ weight and histopathological examination. From the results, Alginase$Alginase^{ⓡ}$ seems not to have any toxic effect in rats when it were given daily intravenous injections below the dosage 1.25U/kg B.W./day for four weeks.

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